Rates of homology directed repair of CRISPR-Cas9 induced double strand breaks are lower in naïve compared to primed human pluripotent stem cells.

Dodsworth, Benjamin T; Hatje, Klas; Meyer, Claas Aiko; Flynn, Rowan; Cowley, Sally A

Dodsworth, Benjamin T; Hatje, Klas; Meyer, Claas Aiko; Flynn, Rowan; Cowley, Sally A.

Afiliação

Dodsworth BT; Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE, United Kingdom; Roche Pharma Research and Early Development, Roche Innovation Center Basel, Basel, Switzerland.
Hatje K; Roche Pharma Research and Early Development, Roche Innovation Center Basel, Basel, Switzerland.
Meyer CA; Roche Pharma Research and Early Development, Roche Innovation Center Basel, Basel, Switzerland.
Flynn R; Censo Biotechnologies, Roslin Innovation Centre Charnock Bradley Building, Easter Bush Campus EH25 9RG, United Kingdom.
Cowley SA; Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE, United Kingdom. Electronic address: sally.cowley@path.ox.ac.uk.

Stem Cell Res ; 46: 101852, 2020 07.

Article em En | MEDLINE | ID: mdl-32521498

Assuntos

Sistemas CRISPR-Cas; Células-Tronco Pluripotentes; Sistemas CRISPR-Cas/genética; Quebras de DNA de Cadeia Dupla; Reparo do DNA por Junção de Extremidades; Edição de Genes; Humanos; Reparo de DNA por Recombinação

Palavras-chave

Gene editing efficiency; Ground state; HDR; HR; Homologous recombination; Homology directed repair; Human; NHEJ; Naïve; Non-homologous end joining; Pluripotency; Pluripotent; Stem cell

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes / Sistemas CRISPR-Cas Idioma: En Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Suíça

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