Anticoccidial effect of Fructus Meliae toosendan extract against Eimeria tenella.
Pharm Biol
; 58(1): 636-645, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-32634340
ABSTRACT
CONTEXT Fructus Meliae toosendan extracts (FMTE) have a good therapeutic effect on coccidiosis, but there is no relevant research on its prophylactic effect on coccidiosis. OBJECTIVE:
This study comprehensively evaluates the anticoccidial effect of FMTE. MATERIALS ANDMETHODS:
In vitro, the unsporulated oocysts were treated with serial dilutions of FMTE and incubated for 7 d, and the sporulated oocysts were counted for calculating the median lethal concentration (LC50) of FMTE. In vivo, 180 10-day-old broiler chickens free of coccidiosis were weighted and randomly distributed into six groups normal group, untreated group, 4 protective groups (positive group and three FMTE groups). From day 10 to day 21, chickens in the three FMTE groups were pre-treated with FMTE at the dosage of 2.5, 5 and 10 g/kg/d, respectively, and chickens in the positive group were pre-treated with qiuliling (10 g/kg/d). On day 14, chickens in all groups except the normal group were orally infected with 1.5 × 104 sporulated oocysts. The clinical symptoms were observed from day 10 to day 21, the anticoccidial index (ACI), tissue lesions, and intestinal microflora were determined on day 21.RESULTS:
FMTE showed anti-sporulation effect against E. tenella and the LC50 value was 245.83 µg/mL in vitro. In vivo, FMTE at the dosage of 10 g/kg/d was effective against E. tenella infection, and its ACI value was 162.56, which was higher than the value of positive drug qiuliling (128.81). Discussion andconclusions:
FMTE have potent anticoccidial effects, and it presents an alternative anticoccidial agent for avian coccidiosis control.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
/
Extratos Vegetais
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Coccidiose
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Meliaceae
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China