Multiomic analysis of a dried single-drop plasma sample using an integrated mass spectrometry approach.
Analyst
; 145(20): 6441-6446, 2020 Oct 21.
Article
em En
| MEDLINE
| ID: mdl-32785396
An easy-to-use and fast approach was developed for integrated proteomic and metabolic profiling in a dried single-drop plasma sample. Plasma collection, room temperature storage, and sample preparation for both proteins and metabolites were seamlessly integrated in one spintip device. MS-based multiomic profiling using the same nano LC-MS system identified more than 150 proteins and 160 metabolites from the 1 µL plasma sample in 6 hours. Further combination with micro-flow LC and targeted MS made it a promising approach for the fast profiling of molecular biomarkers with high sensitivity and accuracy.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteômica
/
Espectrometria de Massas em Tandem
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China