Intramolecular Photoelectrochemical System Using Tyrosine-Modified Antibody-Targeted Peptide as Electron Donor for Detection of Biomarkers.
Anal Chem
; 92(16): 10935-10939, 2020 08 18.
Article
em En
| MEDLINE
| ID: mdl-32806903
ABSTRACT
An intramolecular photoelectrochemical (PEC) system is designed from the novel electron donor YYYHWRGWV (Y3-H) peptide ligand for the first time. The bifunctional nonapeptide cannot only rely on the HWRGWV sequence as a site-oriented immobilizer to recognize the crystallizable fragment (Fc) domains of the antibody but also acts as electron donors for PEC generation via three tyrosine (Y) of the N-terminal. The Bi2WO6/AgInS2 heterojunction with a significant visible-light absorption is utilized as a photoelectric generator, and the motivation is ascribed to a proven proposition, namely, that short-wavelength illuminant radiates proteins, causing a decline in bioactivity of immune protein. An innovative biosensor is fabricated using the above strategies for the detection of CYFRA21-1, a biomarker of squamous cell lung carcinoma. This sort of PEC-based sensing platform shows convincing experimental data and could be an effective candidate for clinical application in the future due to their extremely skillful conception.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
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Tirosina
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Técnicas Biossensoriais
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Biomarcadores Tumorais
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Queratina-19
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Técnicas Eletroquímicas
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Antígenos de Neoplasias
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article