Quantitative mass spectrometry-based analysis of proteins related to cattle and their products - Focus on cows' milk beta-casein proteoforms.
Methods
; 186: 112-118, 2021 02.
Article
em En
| MEDLINE
| ID: mdl-32956783
ABSTRACT
Modern mass spectrometers can accurately measure thousands of compounds in complex mixtures over a given liquid chromatograph method, depending on desired outcome and method duration. This stream of analytical chemistry has wide ranging application across food, pharma, environmental, forensics, clinical and research. With consistent pressure on both the ruminant production and product industries to face new and substantial challenges, liquid chromatography-mass spectrometry (LC-MS) is an ideal tool to identify, detect and quantify markers of breeding, production and adaption to support both research and industry to overcome these challenges. Herein, we provide a description of the theoretical basis and framework for LC-MS as a rapidly developing technique and highlight its application in measuring cattle and cattle product traits through protein quantitation with specific focus on beta-casein proteoforms.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Caseínas
/
Indústria de Laticínios
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Leite
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Espectrometria de Massas em Tandem
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article