Clickable gold-nanoparticles as generic probe precursors for facile photoaffinity labeling application.
Org Biomol Chem
; 19(6): 1268-1273, 2021 02 18.
Article
em En
| MEDLINE
| ID: mdl-33331841
ABSTRACT
Rapid access to appropriately functionalized probes is crucial in chemical labeling approaches to target identification studies. We designed and synthesized clickable gold-nanoparticles as generic probe precursors that enable (1) one-step ligand derivatization by click chemistry, and (2) facile photoaffinity labeling application. Using cholesterol as a model ligand, we successfully demonstrated the utility of the ligand-clicked probe in photoaffinity labeling of endogenously expressed oxysterol-binding protein (OSBP) in cell lysate.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Marcadores de Fotoafinidade
/
Nanopartículas Metálicas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Japão