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CD133+/CD166+ human gastric adenocarcinoma cells present the properties of neoplastic stem cells and emerge more malignant features.
Ni, Tengyang; Wang, Haibo; Zhan, Dongmei; Tao, Li; Lv, Mengying; Wang, Weimin; Chu, Zewen; Zhou, Zhen; Sunagawa, Masataka; Liu, Yanqing.
Afiliação
  • Ni T; Institute of Translational Medicine, Medical College, Yangzhou University, The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou 225001, China.
  • Wang H; Institute of Translational Medicine, Medical College, Yangzhou University, The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou 225001, China.
  • Zhan D; Institute of Translational Medicine, Medical College, Yangzhou University, The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou 225001, China.
  • Tao L; Department of Pharmacy, College of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, China.
  • Lv M; Department of Pharmacy, College of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, China.
  • Wang W; Institute of Translational Medicine, Medical College, Yangzhou University, The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou 225001, China.
  • Chu Z; Institute of Translational Medicine, Medical College, Yangzhou University, The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou 225001, China.
  • Zhou Z; Institute of Translational Medicine, Medical College, Yangzhou University, The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou 225001, China.
  • Sunagawa M; Department of Physiology, School of Medicine, Showa University, Tokyo 142, Japan.
  • Liu Y; Institute of Translational Medicine, Medical College, Yangzhou University, The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou 225001, China. Electronic address: liuyq@yzu.edu.cn.
Life Sci ; 269: 119021, 2021 Mar 15.
Article em En | MEDLINE | ID: mdl-33450261
AIMS: The recurrence and metastasis of gastric cancer has always been an important factor affecting the prognosis of gastric cancer. Cancer stem cells can promote the recurrence and growth of gastric cancer. The identification and isolation of gastric cancer stem cells contribute to the origin, progress and treatment strategy of gastric cancer. The aim of this study was to identify and isolate gastric cancer stem cells, and provide targets for the treatment of gastric cancer. METHODS: Magnetic-activated cell sorting was used to isolate CD133+/CD166+ cell populations from human gastric adenocarcinoma cell lines (BGC-823 and SGC-7901). Sphere formation, cell proliferation, resistance to chemotherapy, colony formation, migration invasion and tumorigenicity in vivo of these cell populations were evaluated. Moreover, RT-qPCR and Western blot were used to investigate the expression level of the stem cell markers Nanog, Sox2, Oct-4, and c-Myc. RESULTS: CD133+/CD166+ cell subpopulations presented more malignant features than CD133-/CD166-, CD133-/CD166+, CD133+/CD166- cell populations and parental cells. Moreover, the mRNA and protein expression level of Oct-4 and c-Myc were higher in CD133+/CD166+ cells than in parental cells or other cell populations. CONCLUSION: The CD133+/CD166+ populations of human gastric cancer cell lines BGC-823 and SGC-7901 have cancer stem cell characteristics.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Células-Tronco Neoplásicas / Adenocarcinoma / Molécula de Adesão de Leucócito Ativado / Antígeno AC133 Idioma: En Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Células-Tronco Neoplásicas / Adenocarcinoma / Molécula de Adesão de Leucócito Ativado / Antígeno AC133 Idioma: En Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China