Membrane Protein Production and Purification from Escherichia coli and Sf9 Insect Cells.

Liu, Yixin; Pavic, Ana; Farley, Joshua T; de Marcos Lousa, Carine; Goldman, Adrian; Postis, Vincent L G

Liu, Yixin; Pavic, Ana; Farley, Joshua T; de Marcos Lousa, Carine; Goldman, Adrian; Postis, Vincent L G.

Afiliação

Liu Y; Molecular and Integrative Biosciences, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland.
Pavic A; Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, UK.
Farley JT; School of Clinical and Applied Sciences, Leeds Beckett University, Leeds, UK.
de Marcos Lousa C; School of Clinical and Applied Sciences, Leeds Beckett University, Leeds, UK.
Goldman A; School of Clinical and Applied Sciences, Leeds Beckett University, Leeds, UK.
Postis VLG; Molecular and Integrative Biosciences, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland.

Methods Mol Biol ; 2168: 3-49, 2020.

Article em En | MEDLINE | ID: mdl-33582985

Assuntos

Cromatografia de Afinidade/métodos; Escherichia coli/metabolismo; Proteínas de Fluorescência Verde/metabolismo; Proteínas de Membrana/isolamento & purificação; Proteínas de Membrana/metabolismo; Animais; Escherichia coli/crescimento & desenvolvimento; Vetores Genéticos; Humanos; Células Sf9

Palavras-chave

Affinity chromatography; Bacterial expression; Baculovirus-infected insect cells; Green fluorescence protein; In-gel fluorescence; Membrane protein; Protein expression; Protein purification

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cromatografia de Afinidade / Proteínas de Fluorescência Verde / Escherichia coli / Proteínas de Membrana Idioma: En Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Finlândia

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