[Quantitation and content investigation of bilirubin in cultured cow-bezoar].
Zhongguo Zhong Yao Za Zhi
; 46(11): 2852-2856, 2021 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-34296585
ABSTRACT
A determination method for bilirubin in cultured cow-bezoar was developed in this study, with which the bilirubin in 15 batches of samples was quantified. The samples were first processed with 10% oxalic acid solution for the conversion of bilirubin from conjugated to unconjugated, followed by the extraction with dichloromethane. Then the obtained sample solutions were analyzed at 450 nm by HPLC[chromatographic column Agilent TC-C_(18)(4.6 mm × 250 mm, 5 µm); mobile phase acetonitrile and 1% glacial acetic acid aqueous solution(95â¶5); flow rate 1.0 mL·min~(-1)]. The bilirubin content in the 15 batches of cultured cow-bezoar was ranged from 21.9% to 41.7% with the average of 32.4%. The proposed method is accurate and reliable, thus making it suitable for the quantitation of bilirubin in cultured cow-bezoar and its quality assessment and control.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bezoares
/
Medicamentos de Ervas Chinesas
Idioma:
Zh
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China