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Embryonic stem cells are devoid of macropinocytosis, a trafficking pathway for activin A in differentiated cells.
Kostopoulou, Nikoleta; Bellou, Sofia; Bagli, Eleni; Markou, Maria; Kostaras, Eleftherios; Hyvönen, Marko; Kalaidzidis, Yiannis; Papadopoulos, Angelos; Chalmantzi, Varvara; Kyrkou, Athena; Panopoulou, Ekaterini; Fotsis, Theodore; Murphy, Carol.
Afiliação
  • Kostopoulou N; Foundation for Research & Technology-Hellas (FORTH), Institute of Molecular Biology and Biotechnology (IMBB), Department of Biomedical Research, Ioannina, 45110, Greece.
  • Bellou S; Foundation for Research & Technology-Hellas (FORTH), Institute of Molecular Biology and Biotechnology (IMBB), Department of Biomedical Research, Ioannina, 45110, Greece.
  • Bagli E; Confocal Laser Scanning Microscopy Unit, Network of Research Supporting Laboratories, University of Ioannina, Ioannina, 45110, Greece.
  • Markou M; Foundation for Research & Technology-Hellas (FORTH), Institute of Molecular Biology and Biotechnology (IMBB), Department of Biomedical Research, Ioannina, 45110, Greece.
  • Kostaras E; Foundation for Research & Technology-Hellas (FORTH), Institute of Molecular Biology and Biotechnology (IMBB), Department of Biomedical Research, Ioannina, 45110, Greece.
  • Hyvönen M; Laboratory of Biological Chemistry, University of Ioannina Medical School, Ioannina, 45110, Greece.
  • Kalaidzidis Y; Foundation for Research & Technology-Hellas (FORTH), Institute of Molecular Biology and Biotechnology (IMBB), Department of Biomedical Research, Ioannina, 45110, Greece.
  • Papadopoulos A; Laboratory of Biological Chemistry, University of Ioannina Medical School, Ioannina, 45110, Greece.
  • Chalmantzi V; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1TN, UK.
  • Kyrkou A; Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
  • Panopoulou E; Foundation for Research & Technology-Hellas (FORTH), Institute of Molecular Biology and Biotechnology (IMBB), Department of Biomedical Research, Ioannina, 45110, Greece.
  • Fotsis T; School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
  • Murphy C; Foundation for Research & Technology-Hellas (FORTH), Institute of Molecular Biology and Biotechnology (IMBB), Department of Biomedical Research, Ioannina, 45110, Greece.
J Cell Sci ; 134(13)2021 07 01.
Article em En | MEDLINE | ID: mdl-34313314
Ligand-receptor complexes formed at the plasma membrane are internalised via various endocytic pathways that influence the ultimate signalling output by regulating the selection of interaction partners by the complex along the trafficking route. We report that, in differentiated cells, activin A-receptor complexes are internalised via clathrin-mediated endocytosis (CME) and macropinocytosis (MP), whereas in human embryonic stem cells (hESCs) internalisation occurs via CME. We further show that hESCs are devoid of MP, which becomes functional upon differentiation towards endothelial cells through mesoderm mediators. Our results reveal, for the first time, that MP is an internalisation route for activin A in differentiated cells, and that MP is not active in hESCs and is induced as cells differentiate.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ativinas / Células Endoteliais Idioma: En Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Grécia
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ativinas / Células Endoteliais Idioma: En Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Grécia