Novel PhoH-encoding vibriophages with lytic activity against environmental Vibrio strains.
Arch Microbiol
; 203(9): 5321-5331, 2021 Nov.
Article
em En
| MEDLINE
| ID: mdl-34379161
ABSTRACT
Cholera is a devastating diarrheal disease that accounts for more than 10% of children's lives worldwide, but its treatment is hampered by a rise in antibiotic resistance. One promising alternative to antibiotic therapy is the use of bacteriophages to treat antibiotic-resistant cholera infections, and control Vibrio cholera in clinical cases and in the environment, respectively. Here, we report four novel, closely related environmental myoviruses, VP4, VP6, VP18, and VP24, which we isolated from two environmental toxigenic Vibrio cholerae strains from river Kuja and Usenge beach in Kenya. High-throughput sequencing followed by bioinformatics analysis indicated that the genomes of the four bacteriophages have closely related sequences, with sizes of 148,180 bp, 148,181 bp, 148,179 bp, and 148,179 bp, and a G + C content of 36.4%. The four genomes carry the phoH gene, which is overrepresented in marine cyanophages. The isolated phages displayed a lytic activity against 15 environmental, as well as one clinical, Vibrio cholerae strains. Thus, these novel lytic vibriophages represent potential biocontrol candidates for water decontamination against pathogenic Vibrio cholerae and ought to be considered for future studies of phage therapy.
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Base de dados:
MEDLINE
Assunto principal:
Bacteriófagos
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Vibrio cholerae
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Cólera
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Quênia