The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.
Mol Cell
; 82(3): 542-554.e6, 2022 02 03.
Article
em En
| MEDLINE
| ID: mdl-35081364
ABSTRACT
Non-covalent complexes of glycolytic enzymes, called metabolons, were postulated in the 1970s, but the concept has been controversial. Here we show that a c-Myc-responsive long noncoding RNA (lncRNA) that we call glycoLINC (gLINC) acts as a backbone for metabolon formation between all four glycolytic payoff phase enzymes (PGK1, PGAM1, ENO1, and PKM2) along with lactate dehydrogenase A (LDHA). The gLINC metabolon enhances glycolytic flux, increases ATP production, and enables cell survival under serine deprivation. Furthermore, gLINC overexpression in cancer cells promotes xenograft growth in mice fed a diet deprived of serine, suggesting that cancer cells employ gLINC during metabolic reprogramming. We propose that gLINC makes a functional contribution to cancer cell adaptation and provide the first example of a lncRNA-facilitated metabolon.
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Base de dados:
MEDLINE
Assunto principal:
Fosfoglicerato Quinase
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Fosfopiruvato Hidratase
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Hormônios Tireóideos
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Proteínas de Transporte
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Biomarcadores Tumorais
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Fosfoglicerato Mutase
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Proteínas Supressoras de Tumor
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Proteínas de Ligação a DNA
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RNA Longo não Codificante
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Glicólise
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article