Structure and mechanism of a methyltransferase ribozyme.
Nat Chem Biol
; 18(5): 556-564, 2022 05.
Article
em En
| MEDLINE
| ID: mdl-35301479
ABSTRACT
Known ribozymes in contemporary biology perform a limited range of chemical catalysis, but in vitro selection has generated species that catalyze a broader range of chemistry; yet, there have been few structural and mechanistic studies of selected ribozymes. A ribozyme has recently been selected that can catalyze a site-specific methyl transfer reaction. We have solved the crystal structure of this ribozyme at a resolution of 2.3 Å, showing how the RNA folds to generate a very specific binding site for the methyl donor substrate. The structure immediately suggests a catalytic mechanism involving a combination of proximity and orientation and nucleobase-mediated general acid catalysis. The mechanism is supported by the pH dependence of the rate of catalysis. A selected methyltransferase ribozyme can thus use a relatively sophisticated catalytic mechanism, broadening the range of known RNA-catalyzed chemistry.
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1
Base de dados:
MEDLINE
Assunto principal:
RNA Catalítico
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China