"X" marks the spot: Mining the gold in CasX for gene editing.
Mol Cell
; 82(6): 1083-1085, 2022 03 17.
Article
em En
| MEDLINE
| ID: mdl-35303481
ABSTRACT
In this issue of Molecular Cell, Tsuchida et al. (2022) present a successful structure-guided effort in improving genome-editing efficiencies of CRISPR-CasX from Deltaproteobacteria (DpbCasX) and Planctomycetes (PlmCasX). Engineered variants that stabilize the active conformational state improved the catalytic efficiency by â¼10-20 fold in vitro and mean-editing efficiency by â¼2-3 fold in human cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sistemas CRISPR-Cas
/
Edição de Genes
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Estados Unidos