Preventive aerobic training preserves sympathovagal function and improves DNA repair capacity of peripheral blood mononuclear cells in rats with cardiomyopathy.

ABSTRACT

To evaluate the effect of preventive aerobic exercise training on sympathovagal function, cardiac function, and DNA repair capacity in a preclinical model of doxorubicin (DOX)-induced cardiomyopathy. Forty male Wistar-Kyoto rats were allocated into four groups (n = 10/group) D (DOX-treated) and C (controls) remained sedentary, and DT (DOX-trained) and CT (control-trained) performed aerobic training 4 days/week, during 4 weeks before exposure to DOX (4 mg/kg/week during 4 weeks) or saline solution. We evaluated cardiac function (echocardiography), hemodynamic and sympathovagal modulation (artery-femoral cannulation), cardiac troponin T levels, and DNA repair capacity (comet assay). Exercise training preserved ejection fraction (D - 14.44% vs. DT - 1.05%, p < 0.001), fractional shortening (D - 8.96% vs. DT - 0.27%, p = 0.025) and troponin T levels (D 6.4 ± 3.6 vs. DT 2.8 ± 1.7 ng/mL, p = 0.010). DOX increased heart rate variability (C 27.7 ± 7.9 vs. D 7.5 ± 2.2 ms2, p < 0.001) and induced sympathovagal dysfunction (LF/HF, C 0.37 ± 0.15 vs. D 0.15 ± 0.15, p = 0.036) through exacerbation of sympathetic function (LF, C 0.22 ± 0.01 vs. D 0.48 ± 0.24 Hz, p = 0.019). Peripheral mononuclear blood cells of DT animals presented lower residual DNA damage (D 43.4 ± 8.4% vs. DT 26 ± 3.4%, p = 0.003 after 1 h). Cardioprotective effects of preventive aerobic exercise training are mediated by preservation of sympathovagal function and improvement of DNA repair capacity of peripheral blood mononuclear cells.