Identification of TCR repertoires in asymptomatic COVID-19 patients by single-cell T-cell receptor sequencing.
Blood Cells Mol Dis
; 97: 102678, 2022 11.
Article
em En
| MEDLINE
| ID: mdl-35716403
ABSTRACT
The T cell-mediated immune responses associated with asymptomatic infection (AS) of SARS-CoV-2 remain largely unknown. The diversity of T-cell receptor (TCR) repertoire is essential for generating effective immunity against viral infections in T cell response. Here, we performed the single-cell TCR sequencing of the PBMC samples from five AS subjects, 33 symptomatic COVID-19 patients and eleven healthy controls to investigate the size and the diversity of TCR repertoire. We subsequently analyzed the TCR repertoire diversity, the V and J gene segment deference, and the dominant combination of αß VJ gene pairing among these three study groups. Notably, we revealed significant TCR preference in the AS group, including the skewed usage of TRAV1-2-J33-TRBV6-4-J2-2 and TRAV1-2-J33-TRBV6-1-J2-3. Our findings may shed new light on understanding the immunopathogenesis of COVID-19 and help identify optimal TCRs for development of novel therapeutic strategies against SARS-CoV-2 infection.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
COVID-19
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China