Genomic hybrid capture assay to detect Borrelia burgdorferi: an application to diagnose neuroborreliosis in horses.
J Vet Diagn Invest
; 34(5): 909-912, 2022 Sep.
Article
em En
| MEDLINE
| ID: mdl-35864735
ABSTRACT
Antemortem diagnosis of neuroborreliosis in horses has been hindered by both the low sensitivity of PCR testing for Borrelia burgdorferi in CSF and the low specificity of serumCSF ELISA ratios used to determine intrathecal antibody production against the bacterium. PCR testing of the CSF of an adult horse with acute neurologic disease for the B. burgdorferi flagellin gene was negative. However, we enriched B. burgdorferi DNA through nucleic acid hybrid capture, followed by next-generation sequencing, and identified B. burgdorferi in the CSF of the horse, confirming a diagnosis of neuroborreliosis.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Doença de Lyme
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Grupo Borrelia Burgdorferi
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Borrelia burgdorferi
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Doenças dos Cavalos
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Doenças do Sistema Nervoso
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Estados Unidos