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Shell microelectrode arrays (MEAs) for brain organoids.
Huang, Qi; Tang, Bohao; Romero, July Carolina; Yang, Yuqian; Elsayed, Saifeldeen Khalil; Pahapale, Gayatri; Lee, Tien-Jung; Morales Pantoja, Itzy E; Han, Fang; Berlinicke, Cynthia; Xiang, Terry; Solazzo, Mallory; Hartung, Thomas; Qin, Zhao; Caffo, Brian S; Smirnova, Lena; Gracias, David H.
Afiliação
  • Huang Q; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
  • Tang B; Department of Biostatistics, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21287, USA.
  • Romero JC; Center for Alternatives to Animal Testing, Department of Environmental Health and Engineering, Bloomberg School of Public Health and Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Yang Y; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
  • Elsayed SK; Department of Civil and Environmental Engineering, Syracuse University, Syracuse, NY 13244, USA.
  • Pahapale G; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
  • Lee TJ; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
  • Morales Pantoja IE; Center for Alternatives to Animal Testing, Department of Environmental Health and Engineering, Bloomberg School of Public Health and Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Han F; Department of Statistics, University of Washington, Seattle, WA 98195, USA.
  • Berlinicke C; Department of Ophthalmology, Johns Hopkins University School of Medicine, Wilmer Eye Institute, Baltimore, MD 21287, USA.
  • Xiang T; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
  • Solazzo M; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
  • Hartung T; Center for Alternatives to Animal Testing, Department of Environmental Health and Engineering, Bloomberg School of Public Health and Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Qin Z; CAAT-Europe, University of Konstanz, 78464 Konstanz, Germany.
  • Caffo BS; Environmental Metrology & Policy Program, Georgetown University, Washington, DC, 20057, USA.
  • Smirnova L; Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, 21205, USA.
  • Gracias DH; Department of Civil and Environmental Engineering, Syracuse University, Syracuse, NY 13244, USA.
Sci Adv ; 8(33): eabq5031, 2022 Aug 19.
Article em En | MEDLINE | ID: mdl-35977026
ABSTRACT
Brain organoids are important models for mimicking some three-dimensional (3D) cytoarchitectural and functional aspects of the brain. Multielectrode arrays (MEAs) that enable recording and stimulation of activity from electrogenic cells offer notable potential for interrogating brain organoids. However, conventional MEAs, initially designed for monolayer cultures, offer limited recording contact area restricted to the bottom of the 3D organoids. Inspired by the shape of electroencephalography caps, we developed miniaturized wafer-integrated MEA caps for organoids. The optically transparent shells are composed of self-folding polymer leaflets with conductive polymer-coated metal electrodes. Tunable folding of the minicaps' polymer leaflets guided by mechanics simulations enables versatile recording from organoids of different sizes, and we validate the feasibility of electrophysiology recording from 400- to 600-µm-sized organoids for up to 4 weeks and in response to glutamate stimulation. Our studies suggest that 3D shell MEAs offer great potential for high signal-to-noise ratio and 3D spatiotemporal brain organoid recording.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos