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Oxidative stress induced by plasma-activated water stimulates astaxanthin production in Phaffia rhodozyma.
Li, Wenshao; Luna-Flores, Carlos H; Anangi, Raveendra; Zhou, Renwu; Tan, Xinle; Jessen, Marius; Liu, Lian; Zhou, Rusen; Zhang, Tianqi; Gissibl, Alexander; Cullen, Patrick J; Ostrikov, Kostya Ken; Speight, Robert E.
Afiliação
  • Li W; School of Chemistry and Physics, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia.
  • Luna-Flores CH; School of Biology and Environmental Science, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia.
  • Anangi R; School of Biology and Environmental Science, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia.
  • Zhou R; School of Chemistry and Physics, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia; School of Chemical and Biomolecular Engineering, The University of Sydney (USYD), Sydney, NSW 2006, Australia; State Key Laboratory of Electrical Insulation and Power Equipment, Centre f
  • Tan X; Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland (UQ), Brisbane, Queensland 4072, Australia.
  • Jessen M; School of Biology and Environmental Science, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia.
  • Liu L; Q-MAP, Metabolomics Australia, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland (UQ), Brisbane, Queensland 4000, Australia.
  • Zhou R; School of Chemistry and Physics, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia; School of Chemical and Biomolecular Engineering, The University of Sydney (USYD), Sydney, NSW 2006, Australia.
  • Zhang T; School of Chemical and Biomolecular Engineering, The University of Sydney (USYD), Sydney, NSW 2006, Australia.
  • Gissibl A; School of Biology and Environmental Science, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia.
  • Cullen PJ; School of Chemical and Biomolecular Engineering, The University of Sydney (USYD), Sydney, NSW 2006, Australia.
  • Ostrikov KK; School of Chemistry and Physics, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia.
  • Speight RE; School of Biology and Environmental Science, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia; ARC Centre of Excellence in Synthetic Biology, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia.
Bioresour Technol ; 369: 128370, 2023 Feb.
Article em En | MEDLINE | ID: mdl-36423765
ABSTRACT
Astaxanthin is used extensively in the nutraceutical, aquaculture, and cosmetic industries. The current market necessitates higher astaxanthin production from Phaffia rhodozyma (P. rhodozyma) due to its higher cost compared to chemical synthesis. In this study, a bubble discharge reactor was developed to generate plasma-activated water (PAW) to produce PAW-made yeast malt (YM) medium. Due to oxidative stress induced by PAW, strains cultured in 15 and 30 min-treated PAW-made medium produced 7.9 ± 1.2 % and 12.6 ± 1.4 % more carotenoids with 15.5 ± 3.3 % and 22.1 ± 1.3 % more astaxanthin, respectively. Reactive oxygen species (ROS) assay results showed that ROS generated by plasma-water interactions elevated intracellular ROS levels. Proteomic analysis revealed increased expression of proteins involved in the cellular response to oxidative stress as well as carotenoid biosynthesis, both of which contribute to higher yields of astaxanthin. Overall, this study supports the potential of PAW to increase astaxanthin yields for industrial-scale production.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Basidiomycota / Proteômica Idioma: En Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Austrália
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Basidiomycota / Proteômica Idioma: En Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Austrália