Hibiscus sabdariffa (Roselle) calyx: a systematic and meta-analytic review of memory-enhancing, anti-neuroinflammatory and antioxidative activities.

BACKGROUND: In this study, we summarized the preclinical investigations of the neuroprotective activities of Hibiscus sabdariffa (HSD) extract via its effect on memory function, neuroinflammation and oxidative damage in the central nervous system, which may help to guide future studies. METHODS: Preclinical studies that investigated the effect of HSD extract on memory impairment, neuroinflammation and oxidative stress-induced neuronal damage were searched systematically in PubMed, EBSCOhost (including MEDLINE, CINAHL, APA PsycInfo, etc.), Web of Science (WoS) and Scopus. Parameters and indexes included Morris water maze, passive avoidance test, acetylcholinesterase activity, interleukin 1 (IL-1), tumour necrosis factor-alpha (TNF-α), MAPK, malondialdehyde (MDA), glutathione (GSH), reactive oxygen species (ROS) and mitochondria membrane potential (MMP). RESULTS: A total of 285 documents were identified; however, only ten articles were included and used for meta-analysis. The meta-analytic outcome revealed that HSD did not show any significant effect on memory function, neuroinflammatory biomarkers (IL-1, MAPK) and oxidative stress (GSH, MDA, ROS and MMP) in neuronal cells and tissues. CONCLUSIONS: Individual study revealed that HSD showed improved memory function, attenuated neuroinflammation and prevented oxidative damage to neurons. However, a conflicting result was observed from the meta-analytic outcomes which showed that HSD has no significant effect on cognitive impairment, neuroinflammation and oxidative stress-induced neuronal damage. However the contradiction in this finding may be associated with small number of studies included. Hence, more studies on the memory-enhacing effects and anti-neuroinflammatory activity of HSD in preclinical and clinical model are required to validate its neuroprotective effect.