Rhinovirus C causes heterogeneous infection and gene expression in airway epithelial cell subsets.
Mucosal Immunol
; 16(4): 386-398, 2023 08.
Article
em En
| MEDLINE
| ID: mdl-36796588
ABSTRACT
Rhinoviruses infect ciliated airway epithelial cells, and rhinoviruses' nonstructural proteins quickly inhibit and divert cellular processes for viral replication. However, the epithelium can mount a robust innate antiviral immune response. Therefore, we hypothesized that uninfected cells contribute significantly to the antiviral immune response in the airway epithelium. Using single-cell RNA sequencing, we demonstrate that both infected and uninfected cells upregulate antiviral genes (e.g. MX1, IFIT2, IFIH1, and OAS3) with nearly identical kinetics, whereas uninfected non-ciliated cells are the primary source of proinflammatory chemokines. Furthermore, we identified a subset of highly infectable ciliated epithelial cells with minimal interferon responses and determined that interferon responses originate from distinct subsets of ciliated cells with moderate viral replication. These findings suggest that the composition of ciliated airway epithelial cells and coordinated responses of infected and uninfected cells could determine the risk of more severe viral respiratory illnesses in children with asthma, chronic obstructive pulmonary disease, and genetically susceptible individuals.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Interferons
/
Células Epiteliais
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article