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One-step CRISPR-Cas9-mediated knockout of native TCRαß genes in human T cells using RNA electroporation.
Flumens, Donovan; Campillo-Davo, Diana; Janssens, Ibo; Roex, Gils; De Waele, Jorrit; Anguille, Sébastien; Lion, Eva.
Afiliação
  • Flumens D; Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, 2610 Wilrijk, Belgium. Electronic address: donovan.flumens@uantwerpen.be.
  • Campillo-Davo D; Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, 2610 Wilrijk, Belgium.
  • Janssens I; Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, 2610 Wilrijk, Belgium.
  • Roex G; Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, 2610 Wilrijk, Belgium.
  • De Waele J; Center for Oncological Research (CORE), Integrated Personalized and Precision Oncology Network (IPPON), 2610 Wilrijk, Belgium.
  • Anguille S; Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, 2610 Wilrijk, Belgium; Division of Hematology, Antwerp University Hospital, Drie Eikenstraat 655, 2650 Edegem, Belgium; Center for Cell The
  • Lion E; Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, 2610 Wilrijk, Belgium; Center for Cell Therapy & Regenerative Medicine, Antwerp University Hospital, 2650 Edegem, Belgium. Electronic a
STAR Protoc ; 4(1): 102112, 2023 03 17.
Article em En | MEDLINE | ID: mdl-36853667
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Receptores de Antígenos de Linfócitos T alfa-beta / Linfócitos T CD8-Positivos Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Receptores de Antígenos de Linfócitos T alfa-beta / Linfócitos T CD8-Positivos Idioma: En Ano de publicação: 2023 Tipo de documento: Article