FAMetA: a mass isotopologue-based tool for the comprehensive analysis of fatty acid metabolism.
Brief Bioinform
; 24(2)2023 03 19.
Article
em En
| MEDLINE
| ID: mdl-36857618
ABSTRACT
The use of stable isotope tracers and mass spectrometry (MS) is the gold standard method for the analysis of fatty acid (FA) metabolism. Yet, current state-of-the-art tools provide limited and difficult-to-interpret information about FA biosynthetic routes. Here we present FAMetA, an R package and a web-based application (www.fameta.es) that uses 13C mass isotopologue profiles to estimate FA import, de novo lipogenesis, elongation and desaturation in a user-friendly platform. The FAMetA workflow covers the required functionalities needed for MS data analyses. To illustrate its utility, different in vitro and in vivo experimental settings are used in which FA metabolism is modified. Thanks to the comprehensive characterization of FA biosynthesis and the easy-to-interpret graphical representations compared to previous tools, FAMetA discloses unnoticed insights into how cells reprogram their FA metabolism and, when combined with FASN, SCD1 and FADS2 inhibitors, it enables the identification of new FAs by the metabolic reconstruction of their synthesis route.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Metabolismo dos Lipídeos
/
Lipogênese
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Espanha