Enhanced production of aspochalasin D through genetic engineering of Aspergillus flavipes.
Appl Microbiol Biotechnol
; 107(9): 2911-2920, 2023 May.
Article
em En
| MEDLINE
| ID: mdl-37004567
ABSTRACT
Aspochalasin D (AD) belongs to the polyketide-amino acid hybrid natural products with anti-cancer, anti-bacterial, and anti-fouling bioactivities. However, the low production limits its further application. In this study, AD was separated and identified from Aspergillus flavipes 3.17641. Next, besides the optimization of culture conditions using a single-factor experiment and response surface methodology, metabolic engineering was employed to increase the AD production. It shows that the deletion of the shunt gene aspoA and overexpression of the pathway-specific regulator aspoG significantly improve the AD production. Its production reached to 812.1 mg/L under the optimized conditions, with 18.5-fold increase. Therefore, this study not only provides a general method for improving the production of similar natural products in other fungi, but also enables the further biological function development of AD in agriculture and pharmaceutical. KEY POINTS ⢠The Aspochalasin D (AD) production was improved by optimizing culture conditions. ⢠The deletion of the shunt gene aspoA increased the AD production. ⢠Overexpression of the pathway regulator aspoG further improved the AD production.
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Base de dados:
MEDLINE
Assunto principal:
Aspergillus
/
Produtos Biológicos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China