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Biological differences between FIM2 and FIM3 fimbriae of Bordetella pertussis: not just the serotype.
Matczak, Soraya; Bouchez, Valérie; Leroux, Pauline; Douché, Thibaut; Collinet, Nils; Landier, Annie; Gianetto, Quentin Giai; Guillot, Sophie; Chamot-Rooke, Julia; Hasan, Milena; Matondo, Mariette; Brisse, Sylvain; Toubiana, Julie.
Afiliação
  • Matczak S; Institut Pasteur, Université Paris Cité, Biodiversity and Epidemiology of Bacterial Pathogens, 28, Rue Du Docteur Roux, 75015, Paris, France.
  • Bouchez V; Institut Pasteur, Université Paris Cité, Biodiversity and Epidemiology of Bacterial Pathogens, 28, Rue Du Docteur Roux, 75015, Paris, France; National Reference Center for Whooping Cough and Other Bordetella Infections, Institut Pasteur, 28, Rue Du Docteur Roux, 75015, Paris, France.
  • Leroux P; Institut Pasteur, Université Paris Cité, Biodiversity and Epidemiology of Bacterial Pathogens, 28, Rue Du Docteur Roux, 75015, Paris, France.
  • Douché T; Institut Pasteur, Université Paris Cité, CNRS UAR2024, Proteomics Platform, Mass Spectrometry for Biology Unit, 28, Rue Du Docteur Roux, 75015, Paris, France.
  • Collinet N; Institut Pasteur, Université Paris Cité, Biodiversity and Epidemiology of Bacterial Pathogens, 28, Rue Du Docteur Roux, 75015, Paris, France.
  • Landier A; Institut Pasteur, Université Paris Cité, Biodiversity and Epidemiology of Bacterial Pathogens, 28, Rue Du Docteur Roux, 75015, Paris, France; National Reference Center for Whooping Cough and Other Bordetella Infections, Institut Pasteur, 28, Rue Du Docteur Roux, 75015, Paris, France.
  • Gianetto QG; Institut Pasteur, Université Paris Cité, CNRS UAR2024, Proteomics Platform, Mass Spectrometry for Biology Unit, 28, Rue Du Docteur Roux, 75015, Paris, France; Institut Pasteur, Université Paris Cité, Bioinformatics and Biostatistics Hub, 28, Rue Du Docteur Roux, 75015, Paris, France.
  • Guillot S; Institut Pasteur, Université Paris Cité, Biodiversity and Epidemiology of Bacterial Pathogens, 28, Rue Du Docteur Roux, 75015, Paris, France; National Reference Center for Whooping Cough and Other Bordetella Infections, Institut Pasteur, 28, Rue Du Docteur Roux, 75015, Paris, France.
  • Chamot-Rooke J; Institut Pasteur, Université Paris Cité, CNRS UAR2024, Proteomics Platform, Mass Spectrometry for Biology Unit, 28, Rue Du Docteur Roux, 75015, Paris, France.
  • Hasan M; Institut Pasteur, Université Paris Cité, Cytometry and Biomarkers Unit of Technology and Service (CB UTechS), 28, Rue Du Docteur Roux, 75015, Paris, France.
  • Matondo M; Institut Pasteur, Université Paris Cité, CNRS UAR2024, Proteomics Platform, Mass Spectrometry for Biology Unit, 28, Rue Du Docteur Roux, 75015, Paris, France.
  • Brisse S; Institut Pasteur, Université Paris Cité, Biodiversity and Epidemiology of Bacterial Pathogens, 28, Rue Du Docteur Roux, 75015, Paris, France; National Reference Center for Whooping Cough and Other Bordetella Infections, Institut Pasteur, 28, Rue Du Docteur Roux, 75015, Paris, France.
  • Toubiana J; Institut Pasteur, Université Paris Cité, Biodiversity and Epidemiology of Bacterial Pathogens, 28, Rue Du Docteur Roux, 75015, Paris, France; National Reference Center for Whooping Cough and Other Bordetella Infections, Institut Pasteur, 28, Rue Du Docteur Roux, 75015, Paris, France; Department of G
Microbes Infect ; 25(7): 105152, 2023.
Article em En | MEDLINE | ID: mdl-37245862
INTRODUCTION: Bordetella pertussis still circulates worldwide despite vaccination. Fimbriae are components of some acellular pertussis vaccines. Population fluctuations of B. pertussis fimbrial serotypes (FIM2 and FIM3) are observed, and fim3 alleles (fim3-1 [clade 1] and fim3-2 [clade 2]) mark a major phylogenetic subdivision of B. pertussis. OBJECTIVES: To compare microbiological characteristics and expressed protein profiles between fimbrial serotypes FIM2 and FIM3 and genomic clades. METHODS: A total of 19 isolates were selected. Absolute protein abundance of the main virulence factors, autoagglutination and biofilm formation, bacterial survival in whole blood, induced blood cell cytokine secretion, and global proteome profiles were assessed. RESULTS: Compared to FIM3, FIM2 isolates produced more fimbriae, less cellular pertussis toxin subunit 1 and more biofilm, but auto-agglutinated less. FIM2 isolates had a lower survival rate in cord blood, but induced higher levels of IL-4, IL-8 and IL-1ß secretion. Global proteome comparisons uncovered 15 differentially produced proteins between FIM2 and FIM3 isolates, involved in adhesion and metabolism of metals. FIM3 isolates of clade 2 produced more FIM3 and more biofilm compared to clade 1. CONCLUSION: FIM serotype and fim3 clades are associated with proteomic and other biological differences, which may have implications on pathogenesis and epidemiological emergence.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bordetella pertussis / Coqueluche Idioma: En Ano de publicação: 2023 Tipo de documento: Article País de afiliação: França
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bordetella pertussis / Coqueluche Idioma: En Ano de publicação: 2023 Tipo de documento: Article País de afiliação: França