Optimization of a medium composition for the heterologous production of Alcaligenes faecalis penicillin G acylase in Bacillus megaterium.

Lopes, Wagner; Deolindo, Poliana; de Souza Costa, Alexandre Andrade; Gomes da Silva, Melissa Teixeira; de Miranda, Otavio Padula; Pacheco, Graziela Jardim

Lopes, Wagner; Deolindo, Poliana; de Souza Costa, Alexandre Andrade; Gomes da Silva, Melissa Teixeira; de Miranda, Otavio Padula; Pacheco, Graziela Jardim.

Afiliação

Lopes W; Institute of Drug Technology, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil.
Deolindo P; Institute of Drug Technology, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil.
de Souza Costa AA; Institute of Drug Technology, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil.
Gomes da Silva MT; Institute of Drug Technology, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil.
de Miranda OP; Institute of Drug Technology, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil.
Pacheco GJ; Institute of Drug Technology, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil. Electronic address: graziela.pacheco@fiocruz.br.

Protein Expr Purif ; 210: 106327, 2023 10.

Article em En | MEDLINE | ID: mdl-37348663

Assuntos

Alcaligenes faecalis; Bacillus megaterium; Penicilina Amidase; Alcaligenes faecalis/genética; Alcaligenes faecalis/metabolismo; Penicilina Amidase/genética; Penicilina Amidase/metabolismo; Antibacterianos; beta-Lactamas

Palavras-chave

Alcaligenes faecalis; Bacillus megaterium; Design of experiments; Expression; Penicillin acylase

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Penicilina Amidase / Bacillus megaterium / Alcaligenes faecalis Idioma: En Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google