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In Vivo Imaging of Nipah Virus Infection in Small Animal Rodent Models.
Johnson, Kendra; Juelich, Terry; Smith, Jennifer; Lee, Benhur; Freiberg, Alexander N.
Afiliação
  • Johnson K; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.
  • Juelich T; Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
  • Smith J; Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
  • Lee B; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Freiberg AN; Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA. anfreibe@utmb.edu.
Methods Mol Biol ; 2682: 149-157, 2023.
Article em En | MEDLINE | ID: mdl-37610580
In vivo imaging system (IVIS) is a powerful tool for the study of infectious diseases, providing the ability to non-invasively follow viral infection in an individual animal over time. Recombinant henipaviruses expressing bioluminescent or fluorescent reporter proteins can be used both to monitor the spatial and temporal progression of Nipah virus (NiV) infection in vivo as well as in ex vivo tissues. Virally produced luciferases react with systemically administered substrate to produce bioluminescence that can then be detected via IVIS imaging, while fluorescent reporters inherently generate detectable fluorescence without a substrate. Here we describe protocols applying bioluminescent or fluorescent reporter expressing recombinant viruses to in vivo or ex vivo imaging of NiV infection.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Orthopoxvirus / Infecções por Henipavirus Idioma: En Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Orthopoxvirus / Infecções por Henipavirus Idioma: En Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos