Assessment of the efficiency of immobilized degrading microorganisms in removing the organochlorine pesticide residues from agricultural soils.

ABSTRACT

To investigate the removal of organochlorine pesticide residues by immobilized degrading microbe, indigenous microorganisms from organochlorine pesticide (OCP)-contaminated soils in Chengdu plain, pot experiments were carried out to evaluate the potential of the immobilized complex microbial specific degrading microbe treated with sodium alginate (SA) composite carrier in decontaminating OCP-contaminated soils, and field experiments were also conducted to investigate the enhanced efficiency of immobilized microbial agents on the dissipation of OCPs in the contaminated plots for different cultivation usage. The results showed that the dissipation rate of OCPs in contaminated soils with initial concentrations of 122.24 µg/kg was 89.94% after the addition of 25 mg of immobilized microbial agents at the end of the 90 days of experiment, which was 6.1% higher than that of the compound microbial agents under the same environmental conditions, and the control group without the addition of microbial agents was only 1.18%, while the concentration of OCPs in contaminated soils with initial concentrations of 203.64 µg/kg only decreased to 65.29 µg/kg after the addition of 20 mg of compound microbial agents. In contrast, the soil concentration of immobilized microbial agent treatment group under the same conditions decreased to 52.15 µg/kg. During the field experiment, the enhanced efficiency of immobilized microbial agents on the degradation of OCPs in different cultivation usage was evidently different, showed that the concentration of OCPs in paddy fields (18.60%) > tea gardens (12.17%) ≥ orchards (11.41%) > vegetable fields (6.21%) ≥ dryland (4.79%), which was especially significant in stress environment. Overall, the immobilization treatment obviously improved the degradation potential of OCPs-specific degrading microbe, and the degree of improvement was related to the metabolic activity of the degrading microbe, the addition amount, remediation time, and habitat conditions.