Developmental trajectories and cooperating genomic events define molecular subtypes of BCR::ABL1-positive ALL.
Blood
; 143(14): 1391-1398, 2024 Apr 04.
Article
em En
| MEDLINE
| ID: mdl-38153913
ABSTRACT
ABSTRACT Distinct diagnostic entities within BCRABL1-positive acute lymphoblastic leukemia (ALL) are currently defined by the International Consensus Classification of myeloid neoplasms and acute leukemias (ICC) "lymphoid only", with BCRABL1 observed exclusively in lymphatic precursors, vs "multilineage", where BCRABL1 is also present in other hematopoietic lineages. Here, we analyzed transcriptomes of 327 BCRABL1-positive patients with ALL (age, 2-84 years; median, 46 years) and identified 2 main gene expression clusters reproducible across 4 independent patient cohorts. Fluorescence in situ hybridization analysis of fluorescence-activated cell-sorted hematopoietic compartments showed distinct BCRABL1 involvement in myeloid cells for these clusters (n = 18/18 vs n = 3/16 patients; P < .001), indicating that a multilineage or lymphoid BCRABL1 subtype can be inferred from gene expression. Further subclusters grouped samples according to cooperating genomic events (multilineage HBS1L deletion or monosomy 7; lymphoid IKZF1-/- or CDKN2A/PAX5 deletions/hyperdiploidy). A novel HSB1L transcript was highly specific for BCRABL1 multilineage cases independent of HBS1L genomic aberrations. Treatment on current German Multicenter Study Group for Adult ALL (GMALL) protocols resulted in comparable disease-free survival (DFS) for multilineage vs lymphoid cluster patients (3-year DFS 70% vs 61%; P = .530; n = 91). However, the IKZF1-/- enriched lymphoid subcluster was associated with inferior DFS, whereas hyperdiploid cases showed a superior outcome. Thus, gene expression clusters define underlying developmental trajectories and distinct patterns of cooperating events in BCRABL1-positive ALL with prognostic relevance.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Fusão bcr-abl
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Alemanha