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Dual phosphorylation of DGK5-mediated PA burst regulates ROS in plant immunity.
Kong, Liang; Ma, Xiyu; Zhang, Chao; Kim, Sung-Il; Li, Bo; Xie, Yingpeng; Yeo, In-Cheol; Thapa, Hem; Chen, Sixue; Devarenne, Timothy P; Munnik, Teun; He, Ping; Shan, Libo.
Afiliação
  • Kong L; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Ma X; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA. Electronic address: xiyu.ma@childrens.harvard.edu.
  • Zhang C; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Kim SI; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Li B; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Xie Y; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Yeo IC; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Thapa H; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Chen S; Department of Biology, University of Mississippi, Oxford, MS 38677, USA.
  • Devarenne TP; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Munnik T; Department of Plant Cell Biology, Green Life Sciences Cluster, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam 1098XH, the Netherlands.
  • He P; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA. Electronic address: pinghemi@umich.edu.
  • Shan L; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA. Electronic address: liboshan@umich.edu.
Cell ; 187(3): 609-623.e21, 2024 Feb 01.
Article em En | MEDLINE | ID: mdl-38244548
ABSTRACT
Phosphatidic acid (PA) and reactive oxygen species (ROS) are crucial cellular messengers mediating diverse signaling processes in metazoans and plants. How PA homeostasis is tightly regulated and intertwined with ROS signaling upon immune elicitation remains elusive. We report here that Arabidopsis diacylglycerol kinase 5 (DGK5) regulates plant pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). The pattern recognition receptor (PRR)-associated kinase BIK1 phosphorylates DGK5 at Ser-506, leading to a rapid PA burst and activation of plant immunity, whereas PRR-activated intracellular MPK4 phosphorylates DGK5 at Thr-446, which subsequently suppresses DGK5 activity and PA production, resulting in attenuated plant immunity. PA binds and stabilizes the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD), regulating ROS production in plant PTI and ETI, and their potentiation. Our data indicate that distinct phosphorylation of DGK5 by PRR-activated BIK1 and MPK4 balances the homeostasis of cellular PA burst that regulates ROS generation in coordinating two branches of plant immunity.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis / Diacilglicerol Quinase / Proteínas de Arabidopsis Idioma: En Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis / Diacilglicerol Quinase / Proteínas de Arabidopsis Idioma: En Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos