Coiled-Coil Interaction Toolbox for Engineering Mammalian Cells.
Methods Mol Biol
; 2774: 31-41, 2024.
Article
em En
| MEDLINE
| ID: mdl-38441756
ABSTRACT
Protein interactions play a crucial role in a variety of biological processes. Therefore, regulation of these interactions has received considerable attention in terms of synthetic biology tool development. Of those, a toolbox of small peptides known as coiled coils (CCs) represents a unique effective tool for mediating protein-protein interactions because their binding specificity and affinity can be designed and controlled. CC peptides have been used as a building module for designing synthetic regulatory circuits in mammalian cells, construction of fast response to a signal, amplification of the response, and localization and regulation of function of diverse proteins. In this chapter, we describe a designed set of CCs used for mammalian cell engineering and provide a protocol for the construction of CC-mediated logic circuits in mammalian cells. Ultimately, these tools could be used for diverse biotechnological and therapeutic applications.
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Base de dados:
MEDLINE
Assunto principal:
Biotecnologia
/
Engenharia Celular
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Eslovênia