Generation of mitochondrial replacement monkeys by female pronucleus transfer.
Zool Res
; 45(2): 292-298, 2024 Mar 18.
Article
em En
| MEDLINE
| ID: mdl-38485499
ABSTRACT
Mutations in mitochondrial DNA (mtDNA) are maternally inherited and have the potential to cause severe disorders. Mitochondrial replacement therapies, including spindle, polar body, and pronuclear transfers, are promising strategies for preventing the hereditary transmission of mtDNA diseases. While pronuclear transfer has been used to generate mitochondrial replacement mouse models and human embryos, its application in non-human primates has not been previously reported. In this study, we successfully generated four healthy cynomolgus monkeys ( Macaca fascicularis) via female pronuclear transfer. These individuals all survived for more than two years and exhibited minimal mtDNA carryover (3.8%-6.7%), as well as relatively stable mtDNA heteroplasmy dynamics during development. The successful establishment of this non-human primate model highlights the considerable potential of pronuclear transfer in reducing the risk of inherited mtDNA diseases and provides a valuable preclinical research model for advancing mitochondrial replacement therapies in humans.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Doenças dos Roedores
/
Doenças Mitocondriais
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China