Analysis of synthetic cellular barcodes in the genome and transcriptome with BARtab and bartools.
Cell Rep Methods
; 4(5): 100763, 2024 May 20.
Article
em En
| MEDLINE
| ID: mdl-38670101
ABSTRACT
Cellular barcoding is a lineage-tracing methodology that couples heritable synthetic barcodes to high-throughput sequencing, enabling the accurate tracing of cell lineages across a range of biological contexts. Recent studies have extended these methods by incorporating lineage information into single-cell or spatial transcriptomics readouts. Leveraging the rich biological information within these datasets requires dedicated computational tools for dataset pre-processing and analysis. Here, we present BARtab, a portable and scalable Nextflow pipeline, and bartools, an open-source R package, designed to provide an integrated end-to-end cellular barcoding analysis toolkit. BARtab and bartools contain methods to simplify the extraction, quality control, analysis, and visualization of lineage barcodes from population-level, single-cell, and spatial transcriptomics experiments. We showcase the utility of our integrated BARtab and bartools workflow via the analysis of exemplar bulk, single-cell, and spatial transcriptomics experiments containing cellular barcoding information.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise de Célula Única
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Sequenciamento de Nucleotídeos em Larga Escala
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Transcriptoma
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Austrália