Ottowia cancrivicina sp. nov., isolated from human stomach.
Int J Syst Evol Microbiol
; 74(5)2024 May.
Article
em En
| MEDLINE
| ID: mdl-38728178
ABSTRACT
A Gram-negative, facultative anaerobic, non-motile and rod-shaped bacterium, designated 10c7w1T, was isolated from a human gastrointestinal tract. Colonies on agar plates were small, circular, smooth and beige. The optimal growth conditions were determined to be 37â°C, pH 7.0-7.5 and 0â% (w/v) NaCl. Comparative analysis of complete 16S rRNA gene sequences revealed that strain 10c7w1T showed the highest sequence similarity of 95.8â% to Ottowia beijingensis MCCC 1A01410T, followed by Ottowia thiooxydans (95.2â%) JCM 11629T. The average amino acid identity values between 10c7w1T and O. beijingensis MCCC 1A01410T and O. thiooxydans JCM 11629T were above 60â% (71.4 and 69.5â%). The average nucleotide identity values between strain 10c7w1T and O. beijingensis MCCC 1A01410T and O. thiooxydans JCM 11629T were 76.9 and 72.5â%, respectively. The dominant fatty acids (≥10â%) were straight chain ones, with summed feature 3 (C16ââ1 ω7c/C16ââ1 ω6c), summed feature 8 (C18ââ1 ω7c/C18ââ1 ω6c) and C16ââ00 being the most abundant. Q-8 was the only respiratory quinone. The major polar lipids of strain 10c7w1T were phosphatidylethanolamine, diphosphatidylglycerol and unknown lipids. The DNA G+C content of strain 10c7w1T was 63.6âmol%. On the basis of phylogenetic, phenotypic and chemotaxonomic data, strain 10c7w1T is considered to represent a novel species within the genus Ottowia, for which the name Ottowia cancrivicina sp. nov. is proposed. The type strain is 10c7w1T (=MCCC 1H01399T=KCTC 92200T).
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
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Estômago
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Composição de Bases
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DNA Bacteriano
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RNA Ribossômico 16S
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Técnicas de Tipagem Bacteriana
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Análise de Sequência de DNA
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Ácidos Graxos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article