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LINE-1 retrotransposons contribute to mouse PV interneuron development.
Bodea, Gabriela O; Botto, Juan M; Ferreiro, Maria E; Sanchez-Luque, Francisco J; de Los Rios Barreda, Jose; Rasmussen, Jay; Rahman, Muhammed A; Fenlon, Laura R; Jansz, Natasha; Gubert, Carolina; Gerdes, Patricia; Bodea, Liviu-Gabriel; Ajjikuttira, Prabha; Da Costa Guevara, Darwin J; Cumner, Linda; Bell, Charles C; Kozulin, Peter; Billon, Victor; Morell, Santiago; Kempen, Marie-Jeanne H C; Love, Chloe J; Saha, Karabi; Palmer, Lucy M; Ewing, Adam D; Jhaveri, Dhanisha J; Richardson, Sandra R; Hannan, Anthony J; Faulkner, Geoffrey J.
Afiliação
  • Bodea GO; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia. gabriela.bodea@gmail.com.
  • Botto JM; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia. gabriela.bodea@gmail.com.
  • Ferreiro ME; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.
  • Sanchez-Luque FJ; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.
  • de Los Rios Barreda J; Institute of Parasitology and Biomedicine 'López-Neyra', Spanish National Research Council, Granada, Spain.
  • Rasmussen J; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.
  • Rahman MA; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.
  • Fenlon LR; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.
  • Jansz N; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.
  • Gubert C; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia.
  • Gerdes P; Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria, Australia.
  • Bodea LG; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia.
  • Ajjikuttira P; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.
  • Da Costa Guevara DJ; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.
  • Cumner L; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.
  • Bell CC; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia.
  • Kozulin P; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.
  • Billon V; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia.
  • Morell S; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.
  • Kempen MHC; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.
  • Love CJ; Biology Department, École Normale Supérieure Paris-Saclay, Gif-sur-Yvette, France.
  • Saha K; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia.
  • Palmer LM; Department of Genetics, University of Cambridge, Cambridge, UK.
  • Ewing AD; MRC Human Genetics Unit, Institute of Genetics and Cancer, University of Edinburgh, Western General Hospital, Edinburgh, UK.
  • Jhaveri DJ; Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria, Australia.
  • Richardson SR; Department of Pharmaceutical Sciences, South Dakota State University, Brookings, SD, USA.
  • Hannan AJ; Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria, Australia.
  • Faulkner GJ; Florey Department of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria, Australia.
Nat Neurosci ; 27(7): 1274-1284, 2024 Jul.
Article em En | MEDLINE | ID: mdl-38773348
ABSTRACT
Retrotransposons are mobile DNA sequences duplicated via transcription and reverse transcription of an RNA intermediate. Cis-regulatory elements encoded by retrotransposons can also promote the transcription of adjacent genes. Somatic LINE-1 (L1) retrotransposon insertions have been detected in mammalian neurons. It is, however, unclear whether L1 sequences are mobile in only some neuronal lineages or therein promote neurodevelopmental gene expression. Here we report programmed L1 activation by SOX6, a transcription factor critical for parvalbumin (PV) interneuron development. Mouse PV interneurons permit L1 mobilization in vitro and in vivo, harbor unmethylated L1 promoters and express full-length L1 mRNAs and proteins. Using nanopore long-read sequencing, we identify unmethylated L1s proximal to PV interneuron genes, including a novel L1 promoter-driven Caps2 transcript isoform that enhances neuron morphological complexity in vitro. These data highlight the contribution made by L1 cis-regulatory elements to PV interneuron development and transcriptome diversity, uncovered due to L1 mobility in this milieu.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Parvalbuminas / Elementos Nucleotídeos Longos e Dispersos / Interneurônios Idioma: En Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Austrália
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Parvalbuminas / Elementos Nucleotídeos Longos e Dispersos / Interneurônios Idioma: En Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Austrália