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Human birth tissue products as a non-opioid medicine to inhibit post-surgical pain.
Zhang, Chi; Huang, Qian; Ford, Neil C; Limjunyawong, Nathachit; Lin, Qing; Yang, Fei; Cui, Xiang; Uniyal, Ankit; Liu, Jing; Mahabole, Megha; He, Hua; Wang, Xue-Wei; Duff, Irina; Wang, Yiru; Wan, Jieru; Zhu, Guangwu; Raja, Srinivasa N; Jia, Hongpeng; Yang, Dazhi; Dong, Xinzhong; Cao, Xu; Tseng, Scheffer C; He, Shao-Qiu; Guan, Yun.
Afiliação
  • Zhang C; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Huang Q; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Ford NC; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Limjunyawong N; The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Lin Q; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Yang F; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Cui X; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Uniyal A; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Liu J; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Mahabole M; BioTissue, Inc., Miami, Florida, USA.
  • He H; BioTissue, Inc., Miami, Florida, USA.
  • Wang XW; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Duff I; Department of Orthopaedic Surgery, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Wang Y; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Wan J; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Zhu G; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Raja SN; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Jia H; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Yang D; Department of Surgery, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Dong X; Acrogenic Technologies Inc., Rockville, Maryland, 20847, USA.
  • Cao X; The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Tseng SC; Howard Hughes Medical Institute, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • He SQ; Department of Orthopaedic Surgery, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205, USA.
  • Guan Y; BioTissue, Inc., Miami, Florida, USA.
bioRxiv ; 2024 Sep 19.
Article em En | MEDLINE | ID: mdl-38826432
ABSTRACT
Pain after surgery causes significant suffering. Opioid analgesics cause severe side effects and accidental death. Therefore, there is an urgent need to develop non-opioid therapies for managing post-surgical pain. Local application of Clarix Flo (FLO), a human amniotic membrane (AM) product, attenuated established post-surgical pain hypersensitivity without exhibiting known side effects of opioid use in mice. This effect was achieved through direct inhibition of nociceptive dorsal root ganglion (DRG) neurons via CD44-dependent pathways. We further purified the major matrix component, the heavy chain-hyaluronic acid/pentraxin 3 (HC-HA/PTX3) from human AM that has greater purity and water solubility than FLO. HC-HA/PTX3 replicated FLO-induced neuronal and pain inhibition. Mechanistically, HC-HA/PTX3 induced cytoskeleton rearrangements to inhibit sodium current and high-voltage activated calcium current on nociceptive neurons, suggesting it is a key bioactive component mediating pain relief. Collectively, our findings highlight the potential of naturally derived biologics from human birth tissues as an effective non-opioid treatment for post-surgical pain. Moreover, we unravel the underlying mechanisms of pain inhibition induced by FLO and HC-HA/PTX3.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos