Single-Cell Synchro-Subtractive-Additive Nanoscale Surgery with Femtosecond Lasers.
Nano Lett
; 24(29): 8801-8808, 2024 Jul 24.
Article
em En
| MEDLINE
| ID: mdl-38989671
ABSTRACT
Herein, an in situ "synchro-subtractive-additive" technique of femtosecond laser single-cell surgery (FLSS) is presented to address the inadequacies of existing surgical methods for single-cell manipulation. This process is enabled by synchronized nanoscale three-dimensional (3D) subtractive and additive manufacturing with ultrahigh precision on various parts of the cells, in that the precise removal and modification of a single-cell structure are realized by nonthermal ablation, with synchronously ultrafast solidification of the specially designed hydrogel by two photopolymerizations. FLSS is a minimally invasive technique with a post-operative survival rate of 70% and stable proliferation. It opens avenues for bottom-up synthetic biology, offering new methods for artificially synthesizing organelle-like 3D structures and modifying the physiological activities of cells.
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Base de dados:
MEDLINE
Assunto principal:
Lasers
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China