Tailor-Made Bioactive Papers by Site-Specific and Orthogonal Covalent Immobilization of Proteins.
Biomacromolecules
; 25(8): 5300-5309, 2024 Aug 12.
Article
em En
| MEDLINE
| ID: mdl-39007485
ABSTRACT
A strategy for the bioorthogonal immobilization of proteins onto commercially available filter paper is presented. Recently, a two-step approach has been described that relies on covalent immobilization of a linker molecule to paper, followed by enzyme-mediated conjugation of a protein of interest containing an enzyme-recognition tag. Here, this strategy was expanded by evaluating four different chemical and chemoenzymatic reactions and investigating paper loading efficiency and orthogonality. Enhanced green fluorescent protein (EGFP) was used as a model protein to allow quantification of protein loading via fluorescence imaging. Two approaches were identified that showed significantly increased loading efficiencies compared with the previously applied conjugation strategy. Additionally, all four methods were proven orthogonal to each other, allowing simultaneous immobilization of a mixture of proteins to a premodified assembly of two paper sheets.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Fluorescência Verde
/
Proteínas Imobilizadas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Alemanha