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Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024.
Schuele, Leonard; Masirika, Leandre Murhula; Udahemuka, Jean Claude; Siangoli, Freddy Belesi; Mbiribindi, Justin Bengehya; Ndishimye, Pacifique; Aarestrup, Frank M; Koopmans, Marion; Oude Munnink, Bas B; Molenkamp, Richard.
Afiliação
  • Schuele L; Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands.
  • Masirika LM; These authors contributed equally to this work and share first authorship.
  • Udahemuka JC; These authors contributed equally to this work and share first authorship.
  • Siangoli FB; Centre de Recherche en Sciences Naturelles de Lwiro, South Kivu, DS Bukavu, Democratic Republic of the Congo.
  • Mbiribindi JB; SaBio Instituto de Investigación en Recursos Cinegéticos IREC (Universidad de Castilla-La Mancha & CSIC), Ciudad Real, Spain.
  • Ndishimye P; Congo Outbreaks, Research for Development, South Kivu, Bukavu, Democratic Republic of the Congo.
  • Aarestrup FM; These authors contributed equally to this work and share first authorship.
  • Koopmans M; Department of Veterinary Medicine, University of Rwanda, Nyagatare, Rwanda.
  • Oude Munnink BB; Genomics Research and Development Division, Stansile Research Organization, Kigali, Rwanda.
  • Molenkamp R; Division Provinciale de la Santé, South Kivu, Bukavu, Democratic Republic of the Congo.
Euro Surveill ; 29(32)2024 Aug.
Article em En | MEDLINE | ID: mdl-39119722
ABSTRACT
Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sensibilidade e Especificidade / Monkeypox virus / Mpox / Reação em Cadeia da Polimerase em Tempo Real Idioma: En Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Holanda
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sensibilidade e Especificidade / Monkeypox virus / Mpox / Reação em Cadeia da Polimerase em Tempo Real Idioma: En Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Holanda