A novel chemiluminescence sensor for alpha-fetoprotein detection based on an aptamer-luminol modified magnetic graphene oxide and copper-based MOF composite.
Anal Methods
; 16(33): 5723-5732, 2024 Aug 22.
Article
em En
| MEDLINE
| ID: mdl-39140150
ABSTRACT
Herein, an aptamer-luminol modified magnetic graphene oxide and copper-based MOF composite was prepared and used to build a novel target-triggered "turn on" chemiluminescence (CL) sensor for alpha-fetoprotein (AFP) detection. Magnetic graphene oxide (MGO) was functionalized with the complementary sequence of the AFP aptamer (cDNA), and then MGO-cDNA was linked to aptamer modified luminol (Apt-luminol) through the complementary base pairing effect. The functionalized magnetic graphene oxide (MGO-cDNA/Apt-luminol) was prepared as a specific magnetic separation and signal switch material. ZnONPs-Au@CuMOFs shows excellent catalytic performance and was used as a catalyst for the luminol-H2O2 reaction. AFP will specifically recognize and bind to Apt on MGO-cDNA/Apt-luminol when AFP is present, which causes luminol release and triggers the CL reaction. The released luminol encounters ZnONPs-Au@CuMOFs and produces strong CL intensity. Therefore, a novel target-triggered "turn on" CL method with high selectivity and sensitivity for detecting AFP has been established. The linear range and detection limit were 1.0 × 10-4-50 ng mL-1 and 4.2 × 10-5 ng mL-1, respectively. The sensor also exhibited good selectivity, reproducibility and stability, and was finally used for AFP detection in serum samples.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Alfa-Fetoproteínas
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Cobre
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Aptâmeros de Nucleotídeos
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Grafite
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Medições Luminescentes
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Luminol
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China