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The modified RNA base acp3U is an attachment site for N-glycans in glycoRNA.
Xie, Yixuan; Chai, Peiyuan; Till, Nicholas A; Hemberger, Helena; Lebedenko, Charlotta G; Porat, Jennifer; Watkins, Christopher P; Caldwell, Reese M; George, Benson M; Perr, Jonathan; Bertozzi, Carolyn R; Garcia, Benjamin A; Flynn, Ryan A.
Afiliação
  • Xie Y; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, USA.
  • Chai P; Stem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
  • Till NA; Department of Chemistry and Sarafan ChEM-H, Stanford University, Stanford, CA, USA.
  • Hemberger H; Stem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
  • Lebedenko CG; Stem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
  • Porat J; Stem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
  • Watkins CP; Stem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
  • Caldwell RM; Stem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
  • George BM; Stem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
  • Perr J; Stem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
  • Bertozzi CR; Department of Chemistry and Sarafan ChEM-H, Stanford University, Stanford, CA, USA; Howard Hughes Medical Institute, Stanford, CA, USA.
  • Garcia BA; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, USA. Electronic address: bagarcia@wustl.edu.
  • Flynn RA; Stem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA; Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA. Electronic address: ryan.flynn@childrens.
Cell ; 187(19): 5228-5237.e12, 2024 Sep 19.
Article em En | MEDLINE | ID: mdl-39173631
ABSTRACT
GlycoRNA consists of RNAs modified with secretory N-glycans that are presented on the cell surface. Although previous work supported a covalent linkage between RNA and glycans, the direct chemical nature of the RNA-glycan connection was not described. Here, we develop a sensitive and scalable protocol to detect and characterize native glycoRNAs. Leveraging RNA-optimized periodate oxidation and aldehyde ligation (rPAL) and sequential window acquisition of all theoretical mass spectra (SWATH-MS), we identified the modified RNA base 3-(3-amino-3-carboxypropyl)uridine (acp3U) as a site of attachment of N-glycans in glycoRNA. rPAL offers sensitivity and robustness as an approach for characterizing direct glycan-RNA linkages occurring in cells, and its flexibility will enable further exploration of glycoRNA biology.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos Idioma: En Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos Idioma: En Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos