Cascading CRISPR/Cas and Nanozyme for Enhanced Organic Photoelectrochemical Transistor Detection with Triple Signal Amplification.
Anal Chem
; 96(35): 14283-14290, 2024 Sep 03.
Article
em En
| MEDLINE
| ID: mdl-39176473
ABSTRACT
Innovative signal amplification and transduction play pivotal roles in bioanalysis. Herein, cascading CRISPR/Cas and the nanozyme are integrated with electronic amplification in an organic photoelectrochemical transistor (OPECT) to enable triple signal amplification, which is exemplified by the miRNA-triggered CRISPR/Cas13a system and polyoxometalate nanozyme for OPECT detection of miRNA-21. The CRISPR/Cas13a-enabled release of glucose oxidase could synergize with peroxidase-like SiW12 to induce catalytic precipitation on the photogate, inhibiting the interfacial mass transfer and thus the significant suppression of the channel current. The as-developed OPECT sensor demonstrates good sensitivity and selectivity for miRNA-21 detection, with a linear range from 1 fM to 10 nM and an ultralow detection limit of 0.53 fM. This study features the integration of bio- and nanoenzyme cascade and electronic triple signal amplification for OPECT detection.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transistores Eletrônicos
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MicroRNAs
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Técnicas Eletroquímicas
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Sistemas CRISPR-Cas
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Glucose Oxidase
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China