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A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic events.
Hao, Yukun A; Lee, Sungmoo; Roth, Richard H; Natale, Silvia; Gomez, Laura; Taxidis, Jiannis; O'Neill, Philipp S; Villette, Vincent; Bradley, Jonathan; Wang, Zeguan; Jiang, Dongyun; Zhang, Guofeng; Sheng, Mengjun; Lu, Di; Boyden, Edward; Delvendahl, Igor; Golshani, Peyman; Wernig, Marius; Feldman, Daniel E; Ji, Na; Ding, Jun; Südhof, Thomas C; Clandinin, Thomas R; Lin, Michael Z.
Afiliação
  • Hao YA; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA.
  • Lee S; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA.
  • Roth RH; Department of Neurosurgery, Stanford University, Stanford, CA 94305, USA.
  • Natale S; Department of Molecular & Cellular Physiology, Stanford University, Stanford, CA 94305, USA.
  • Gomez L; Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, CA 94720, USA; Department of Physics, University of California Berkeley, CA 94720, USA.
  • Taxidis J; Department of Neurology, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA.
  • O'Neill PS; Department of Molecular Life Sciences, University of Zurich (UZH), 8057 Zurich, Switzerland; Neuroscience Center Zurich, 8057 Zurich, Switzerland.
  • Villette V; Institut de Biologie de l'École Normale Supérieure (IBENS), CNRS, INSERM, PSL Research University, Paris 75005, France.
  • Bradley J; Institut de Biologie de l'École Normale Supérieure (IBENS), CNRS, INSERM, PSL Research University, Paris 75005, France.
  • Wang Z; Departments of Brain and Cognitive Sciences, Media Arts and Sciences, and Biological Engineering, MIT, Cambridge, MA 02139, USA; McGovern Institute, MIT, Cambridge, MA 02139, USA.
  • Jiang D; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA.
  • Zhang G; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA.
  • Sheng M; Department of Neurosurgery, Stanford University, Stanford, CA 94305, USA.
  • Lu D; Department of Neurosurgery, Stanford University, Stanford, CA 94305, USA.
  • Boyden E; Departments of Brain and Cognitive Sciences, Media Arts and Sciences, and Biological Engineering, MIT, Cambridge, MA 02139, USA; McGovern Institute, MIT, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA.
  • Delvendahl I; Department of Molecular Life Sciences, University of Zurich (UZH), 8057 Zurich, Switzerland; Neuroscience Center Zurich, 8057 Zurich, Switzerland.
  • Golshani P; Department of Neurology, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA; Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, Los Angeles, CA 90095, USA.
  • Wernig M; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
  • Feldman DE; Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, CA 94720, USA.
  • Ji N; Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, CA 94720, USA; Department of Physics, University of California Berkeley, CA 94720, USA.
  • Ding J; Department of Neurosurgery, Stanford University, Stanford, CA 94305, USA.
  • Südhof TC; Department of Molecular & Cellular Physiology, Stanford University, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Clandinin TR; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA.
  • Lin MZ; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA. Electronic address: mzlin@stanford.edu.
Neuron ; 2024 Sep 17.
Article em En | MEDLINE | ID: mdl-39305894
ABSTRACT
A remaining challenge for genetically encoded voltage indicators (GEVIs) is the reliable detection of excitatory postsynaptic potentials (EPSPs). Here, we developed ASAP5 as a GEVI with enhanced activation kinetics and responsivity near resting membrane potentials for improved detection of both spiking and subthreshold activity. ASAP5 reported action potentials (APs) in vivo with higher signal-to-noise ratios than previous GEVIs and successfully detected graded and subthreshold responses to sensory stimuli in single two-photon trials. In cultured rat or human neurons, somatic ASAP5 reported synaptic events propagating centripetally and could detect ∼1-mV EPSPs. By imaging spontaneous EPSPs throughout dendrites, we found that EPSP amplitudes decay exponentially during propagation and that amplitude at the initiation site generally increases with distance from the soma. These results extend the applications of voltage imaging to the quantal response domain, including in human neurons, opening up the possibility of high-throughput, high-content characterization of neuronal dysfunction in disease.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos