Monoclonal antibody analysis of responder and stimulator cells in the human autologous mixed lymphocyte reaction.
Immunobiology
; 166(2): 190-202, 1984 Mar.
Article
em En
| MEDLINE
| ID: mdl-6233217
ABSTRACT
Responder and stimulator cell subpopulations in the autologous mixed lymphocyte reaction (AMLR) were determined with the OK series of monoclonal antibodies. Mitomycin-C-treated, monocyte-enriched cell populations were used as stimulator cells in the AMLR. Treatment of these monocytes with either OKM and/or OKI monoclonal antibodies and complement resulted in a marked loss of ability of these cells to act as stimulators in the AMLR. Removal of OKT3+ and OKT4+ cells diminished the proliferative responses of AMLR cultures. Interaction of T cells with autologous monocytes resulted in generation of cells capable of suppressing both MLR and AMLR cultures. The suppressor activity of these cells was diminished by treatment with OKI , OKT4 or OKT8 monoclonal antibodies. No cytotoxic activity to autologous or allogeneic monocytes was observed. Additional studies showed an increased number of OKT9 + and OKI + as well as OKT8+ T cells in the AMLR responder cell population. This study indicates that cultures of T lymphocytes with autologous monocytes yield T cell subset(s) which suppress MLR and AMLR reactivity.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos
/
Teste de Cultura Mista de Linfócitos
/
Anticorpos Monoclonais
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article