ATR-FTIR spectroscopic investigation of E. coli transconjugants beta-lactams-resistance phenotype.
FEBS Lett
; 412(1): 39-42, 1997 Jul 21.
Article
em En
| MEDLINE
| ID: mdl-9257685
ABSTRACT
Hyphenation of attenuated total reflection Fourier transform infrared spectroscopy and cluster analysis has been used to characterise a susceptible Escherichia coli K12 strain and the transconjugants TEM-1, TEM-2, TEM-3, SHV-2, SHV-3, SHV-4. A good discrimination of the susceptible strain from the transconjugants was obtained. Although a limited success was achieved in the differentiation of SHV and TEM phenotypes in general, results obtained with TEM-2 and SHV-3 were convincing. Spectral differences observed are ascribed to the global effects of the conjugation process, particularly their repercussions in the nucleic acids and carbohydrate absorbing regions, rather than to beta-lactamase point-mutations.
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Base de dados:
MEDLINE
Assunto principal:
Resistência Microbiana a Medicamentos
/
Espectroscopia de Infravermelho com Transformada de Fourier
/
Conjugação Genética
/
Beta-Lactamas
/
Escherichia coli
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
França