Near-ground effect of height on pollen exposure.

The effect of height on pollen concentration is not well documented and little is known about the near-ground vertical profile of airborne pollen. This is important as most measuring stations are on roofs, but patient exposure is at ground level. Our study used a big data approach to estimate the near-ground vertical profile of pollen concentrations based on a global study of paired stations located at different heights. We analyzed paired sampling stations located at different heights between 1.5 and 50 m above ground level (AGL). This provided pollen data from 59 Hirst-type volumetric traps from 25 different areas, mainly in Europe, but also covering North America and Australia, resulting in about 2,000,000 daily pollen concentrations analyzed. The daily ratio of the amounts of pollen from different heights per location was used, and the values of the lower station were divided by the higher station. The lower station of paired traps recorded more pollen than the higher trap. However, while the effect of height on pollen concentration was clear, it was also limited (average ratio 1.3, range 0.7-2.2). The standard deviation of the pollen ratio was highly variable when the lower station was located close to the ground level (below 10 m AGL). We show that pollen concentrations measured at >10 m are representative for background near-ground levels.

Microscale pollen release and dispersal patterns in flowering grass populations.

Characterizing pollen release and dispersion processes is fundamental for knowledge advancement in ecological, agricultural and public health disciplines. Understanding pollen dispersion from grass communities is especially relevant due to their high species-specific allergenicity and heterogeneously distributed source areas. Here, we aimed to address questions concerning fine level heterogeneity in grass pollen release and dispersion processes, with a focus on characterizing the taxonomic composition of airborne grass pollen over the grass flowering season using eDNA and molecular ecology methods. High resolution grass pollen concentrations were compared between three microscale sites (<300 m apart) in a rural area in Worcestershire, UK. The grass pollen was modelled with local meteorology in a MANOVA (Multivariate ANOVA) approach to investigate factors relevant to pollen release and dispersion. Simultaneously, airborne pollen was sequenced using Illumina MySeq for metabarcoding, analysed against a reference database with all UK grasses using the R packages DADA2 and phyloseq to calculate Shannon's Diversity Index (α-diversity). The flowering phenology of a local Festuca rubra population was observed. We found that grass pollen concentrations varied on a microscale level, likely attributed to local topography and the dispersion distance of pollen from flowering grasses in local source areas. Six genera (Agrostis, Alopecurus, Arrhenatherum, Holcus, Lolium and Poa) dominated the pollen season, comprising on average 77 % of the relative abundance of grass species reads. Temperature, solar radiation, relative humidity, turbulence and wind speeds were found to be relevant for grass pollen release and dispersion processes. An isolated flowering Festuca rubra population contributed almost 40 % of the relative pollen abundance adjacent to the nearby sampler, but only contributed 1 % to samplers situated 300 m away. This suggests that most emitted grass pollen has limited dispersion distance and our results show substantial variation in airborne grass species composition over short geographical scales.

Atmospheric transport reveals grass pollen dispersion distances.

Identifying the origin of bioaerosols is of central importance in many biological disciplines, such as human health, agriculture, forestry, aerobiology and conservation. Modelling sources, transportation pathways and sinks can reveal how bioaerosols vary in the atmosphere and their environmental impact. Grass pollen are particularly important due to their widely distributed source areas, relatively high abundance in the atmosphere and high allergenicity. Currently, studies are uncertain regarding sampler representability between distance and sources for grass pollen. Using generalized linear modelling, this study aimed to analyse this relationship further by answering the question of distance-to-source area contribution. Grass pollen concentrations were compared between urban and rural locations, located 6.4 km apart, during two years in Worcestershire, UK. We isolated and refined vegetation areas at 100 m × 100 m using the 2017 CEH Crop Map and conducted atmospheric modelling using HYSPLIT to identify which source areas could contribute pollen. Pollen concentrations were then modelled with source areas and meteorology using generalized linear mixed-models with three temporal variables as random variation. We found that the Seasonal Pollen Integral for grass pollen varied between both years and location, with the urban location having higher levels. Day of year showed higher temporal variation than the diurnal or annual variables. For the urban location, grass source areas within 30 km had positive significant effects in predicting grass pollen concentrations, while source areas within 2-10 km were important for the rural one. The source area differential was likely influenced by an urban-rural gradient that caused differences in the source area contribution. Temperature had positive highly significant effects on both locations while precipitation affected only the rural location. Combining atmospheric modelling, vegetation source maps and generalized linear modelling was found to be a highly accurate tool to identify transportation pathways of bioaerosols in landscape environments.