ABSTRACT
Lignocellulosic materials form the building block of 50% of plant biomass comprising non-chewable agri-components like wheat straw, rice stubbles, wood shavings and other crop residues. The degradation of lignin, cellulose and hemicellulose is complicated and presently being done by chemical process for industrial application through a very energy intensive process. Lignin degradation is primarily an oxidative process where the enzyme lignin peroxidase digests the polymer into smaller fragments. Being a recalcitrant component, higher lignin content poses a challenge of lower recovery of product for industrial use. Globally, the scientists are working on leveraging fungal biotechnology for using the lignocellulose degrading enzymes secreted by actinomycetes and basidiomycetes fungal groups. Enzymes contributing to degradation of lignin are mainly performing the function of modifying the lignin and degrading the lignin. Ligninolytic enzymes do not act as an independent reaction but are vital to complete the degradation process. Microbial enzyme technology is an emerging green tool in industrial biotechnology for commercial application. Bioprocessing of lignocellulosic biomass is challenged by limitations in enzymatic and conversion process where pretreatment and separation steps are done to remove lignin and hydrolyze carbohydrate into fermentable sugars. This review highlights recent advances in molecular biotechnology, lignin valorization, sequencing, decipher microbial membership, and characterize enzyme diversity through 'omics' techniques. Emerging techniques to characterize the interwoven metabolism and spatial interactions between anaerobes are also reviewed, which will prove critical to developing a predictive understanding of anaerobic communities to guide in microbiome engineering This requires more synergistic collaborations from microbial biotechnologists, bioprocess engineers, enzymologists, and other biotechnological fields.
Subject(s)
Lignin/metabolism , Actinobacteria/metabolism , Adsorption , Bacteria/metabolism , Biodegradation, Environmental , Biotechnology , Fermentation , Fungi/metabolism , MetagenomicsABSTRACT
There has been a spurt in the spread of microbial resistance to antibiotics due to indiscriminate use of antimicrobial agents in human medicine, agriculture, and animal husbandry. It has been realized that conventional antibiotic therapy would be less effective in the coming decades and more emphasis should be given for the development of novel antiinfective therapies. Cysteine rich peptides (CRPs) are broad-spectrum antimicrobial agents that modulate the innate immune system of different life forms such as bacteria, protozoans, fungi, plants, insects, and animals. These are also expressed in several plant tissues in response to invasion by pathogens, and play a crucial role in the regulation of plant growth and development. The present work explores the importance of CRPs as potent antimicrobial agents, which can supplement and/or replace the conventional antibiotics. Different plant parts of diverse plant species showed the presence of antimicrobial peptides (AMPs), which had significant structural and functional diversity. The plant-derived AMPs exhibited potent activity toward a range of plant and animal pathogens, protozoans, insects, and even against cancer cells. The cysteine-rich AMPs have opened new avenues for the use of plants as biofactories for the production of antimicrobials and can be considered as promising antimicrobial drugs in biotherapeutics.
Subject(s)
Anti-Infective Agents/pharmacology , Cysteine/chemistry , Plants/chemistry , Pore Forming Cytotoxic Proteins/pharmacology , Amino Acid Sequence , Animals , Bacteria/drug effects , Humans , Pore Forming Cytotoxic Proteins/chemistry , Protein Structure, SecondaryABSTRACT
The present study envisages biological production of silver nanoparticles using Fusarium oxysporum and in-silico identification of the antibacterial activity of the nanoparticles using protein-ligand interaction studies. The morphology of the nanoparticles was variable, with majority of them spherical in the size range 1-50â¯nm. For in-silico studies, two microorganisms, Escherichia coli and Pseudomonas aeruginosa were selected and metal docking was carried out using the licensed software SYBYL X 1.1.1. The ligand docked deeply into the binding pockets of the outer membrane proteins (OMPs) of both E. coli and P. aeruginosa. The results showed that silver may prove to be a strong antibacterial agent against both the pathogens, with the antibacterial action of silver being greater in the case of P. aeruginosa. The results obtained through in-silico studies were further validated by in-vitro approaches on both solid and liquid media to confirm the results obtained by in-silico analysis. The corroboration of in-silico and in-vitro results amply demonstrates the immense antibacterial potential of silver nanoparticles against the selected pathogens.
Subject(s)
Anti-Bacterial Agents/pharmacology , Escherichia coli/drug effects , Fusarium/metabolism , Metal Nanoparticles , Pseudomonas aeruginosa/drug effects , Silver/pharmacology , Anti-Bacterial Agents/metabolism , Bacterial Outer Membrane Proteins/metabolism , Computer Simulation , Ligands , Protein Binding , Silver/metabolismABSTRACT
A biofilm can be defined as a surface-attached (sessile) community of microorganisms embedded and growing in a self-produced matrix of extracellular polymeric substances. These biofilm communities can be found in medical, industrial and natural environments, and can also be engineered in vitro for various biotechnological applications. Biofilms play a significant role in the transmission and persistence of human disease especially for diseases associated with inert surfaces, including medical devices for internal or external use. Biofilm infections on implants or in-dwelling devices are difficult to eradicate because of their much better protection against macrophages and antibiotics, compared to free living cells, leading to severe clinical complications often with lethal outcome. Recent developments in nanotechnology have provided novel approaches to preventing and dispersing biofilm related infections and potentially providing a novel method for fighting infections that is nondrug related.
Subject(s)
Anti-Infective Agents/pharmacology , Biofilms/drug effects , Prosthesis-Related Infections/prevention & control , Anti-Infective Agents/therapeutic use , Catheter-Related Infections/microbiology , Humans , Nanostructures/therapeutic use , Prosthesis-Related Infections/microbiology , Quorum Sensing/drug effectsABSTRACT
BACKGROUND: Nigella sativa L. has been widely used in the Unani, Ayurveda, Chinese, and Arabic medicine systems and has a long history of medicinal and folk uses. Several phytoconstituents of the plant are reported to have excellent therapeutic properties. In-vitro and in-vivo studies have revealed that seed oil and thymoquinone have excellent inhibitory efficacy on a wide range of both pathogenic and non-pathogenic fungi. OBJECTIVE: The present review aims to undertake a comprehensive and systematic evaluation of the antifungal effects of different phytochemical constituents of black cumin. METHOD: An exhaustive database retrieval was conducted on PubMed, Scopus, ISI Web of Science, SciFinder, Google Scholar, and CABI to collect scientific information about the antifungal activity of N. sativa L. with 1990 to 2023 as a reference range using 'Nigella sativa,' 'Nigella oil,' 'antifungal uses,' 'dermatophytic fungi,' 'candidiasis,' 'anti-aflatoxin,' 'anti-biofilm' and 'biological activity' as the keywords. RESULTS: Black cumin seeds, as well as the extract of aerial parts, were found to exhibit strong antifungal activity against a wide range of fungi. Among the active compounds, thymoquinone exhibited the most potent antifungal effect. Several recent studies proved that black cumin inhibits biofilm formation and growth. CONCLUSION: The review provides an in-depth analysis of the antifungal activity of black cumin. This work emphasizes the need to expand studies on this plant to exploit its antifungal properties for biomedical applications.
Subject(s)
Antifungal Agents , Fungi , Nigella sativa , Antifungal Agents/pharmacology , Antifungal Agents/chemistry , Nigella sativa/chemistry , Fungi/drug effects , Humans , Microbial Sensitivity Tests , Plant Extracts/chemistry , Plant Extracts/pharmacology , Phytochemicals/pharmacology , Phytochemicals/chemistry , Phytochemicals/isolation & purification , Benzoquinones/chemistry , Benzoquinones/pharmacologyABSTRACT
The present investigation was envisaged for large scale in-silico genome wide identification and characterization of glutathione S-transferases (GSTs) in Chenopodium quinoa. In this study, a total of 120 GST genes (CqGSTs) were identified and divided into 11 classes of which tau and phi were highest in numbers. The average protein length of protein was found to be 279.06 with their corresponding average molecular weight of 31,819.4 kDa. The subcellular localization analysis results showed that proteins were centrally localized in the cytoplasm followed by chloroplast, mitochondria and plastids. Structural analysis revealed the presence of 2 -14 exons in CqGST genes. Most of the proteins possessed two exon one intron organization. MEME analysis identified 15 significantly conserved motifs with a width of 6-50 amino acids. Motifs 1, 3, 2, 5, 6, 8, 9 and 13 were found specifically in tau class family; motifs 3, 4, 5, 6, 7 and 9 were found in phi class gene family, while motifs 3, 4, 13 and 14 were found in metaxin class. Multiple sequence alignment revealed highly conserved N-terminus with active site serine (Ser; S) or cysteine (Cys; C) residue for the activation of GSH binding and GST catalytic activity. The gene loci were found to be unevenly distributed across 18 different chromosomes with a maximum of 17 genes located on chromosome number 7. Dominance of alpha helix was followed by coil, extended strand and beta turns. Gene duplication analysis revealed that segmental duplication and purifying type selection were highest in number and found to be main source of expansion of GST gene family. Cis acting regulatory elements analysis showed the presence of 21 different elements involved in stress, hormone and light response and cellular development. The evolutionary relationship of CqGST proteins carried out using maximum likelihood method revealed that all the tau and phi class GSTs were closely associated with those of G. max, O. sativa and A. thaliana. Molecular docking of GST molecules with the fungicide metalaxyl showed that the CqGSTF1 had the lowest binding energy. The comprehensive study of CqGST gene family in quinoa provides groundwork for further functional analysis of CqGST genes in the species at molecular level and has potential applications in plant breeding.
ABSTRACT
The contamination of the environment with toxic metals has become a worldwide problem. Metal toxicity affects crop yields, soil biomass and fertility. Soils polluted with heavy metals pose a serious health hazard to humans as well as plants and animals, and often requires soil remediation practices. Phytoextraction refers to the uptake of contaminants from soil or water by plant roots and their translocation to any harvestable plant part. Phytoextraction has the potential to remove contaminants and promote long-term cleanup of soil or wastewater. The success of phytoextraction as a potential environmental cleanup technology depends on factors like metal availability for uptake, as well as plants ability to absorb and accumulate metals in aerial parts. Efforts are ongoing to understand the genetics and biochemistry of metal uptake, transport and storage in hyperaccumulator plants so as to be able to develop transgenic plants with improved phytoremediation capability. Many plant species are being investigated to determine their usefulness for phytoextraction, especially high biomass crops. The present review aims to give an updated version of information available with respect to metal tolerance and accumulation mechanisms in plants, as well as on the environmental and genetic factors affecting heavy metal uptake. The genetic tools of classical breeding and genetic engineering have opened the door to creation of 'remediation' cultivars. An overview is presented on the possible strategies for developing novel genotypes with increased metal accumulation and tolerance to toxicity.
Subject(s)
Biodegradation, Environmental , Metals, Heavy/metabolism , Plants/metabolism , Soil Pollutants/metabolism , Metals, Heavy/chemistry , Plants/genetics , Soil Pollutants/chemistryABSTRACT
Insulin resistance (IR) is a condition of impaired response of cells towards insulin. It is marked by excessive blood glucose, dysregulated insulin signalling, altered pathways, damaged pancreatic ß-cells, metabolic disorders, etc. Chronic hyperglycemic conditions leads to type 2 diabetes mellitus (T2DM) which causes excess generation of highly reactive free radicals, causing oxidative stress, further leading to development and progression of complications like vascular dysfunction, damaged cellular proteins, and DNA. One of the causes for IR is dysregulation of protein tyrosine phosphatase 1B (PTP1B). Advancements in drug therapeutics have helped people manage IR by regulating PTP1B, however have been reported to cause side effects. Therefore, there is a growing interest on usage of phytochemical constituents having IR therapeutic properties and aiding to minimize these complications. Medicinal plants have not been utilized to their full potential as a therapeutic drug due to lack of knowledge of their active and effective chemical constituents, mode of action, regulation of IR parameters, and dosage of administration. This review highlights phytochemical constituents present in medicinal plants or spices, their potential effectiveness on proteins (PTP1B) regulating IR, and reported possible mechanism of action studied on in vitro models. The study gives current knowledge and future recommendations on the above aspects and is expected to be beneficial in developing herbal drug using these phytochemical constituents, either alone or in combination, for medication of IR and diabetes.
Subject(s)
Diabetes Mellitus, Type 2 , Insulin Resistance , Synthetic Drugs , Blood Glucose , Diabetes Mellitus, Type 2/drug therapy , Humans , Insulin/metabolism , Phosphoric Monoester Hydrolases/therapeutic use , Phytochemicals/pharmacology , Phytochemicals/therapeutic use , Protein Tyrosine Phosphatase, Non-Receptor Type 1/genetics , Synthetic Drugs/therapeutic useABSTRACT
Metal nanoparticles are nanosized entities with dimensions of 1-100 nm that are increasingly in demand due to applications in diverse fields like electronics, sensing, environmental remediation, oil recovery and drug delivery. Metal nanoparticles possess large surface energy and properties different from bulk materials due to their small size, large surface area with free dangling bonds and higher reactivity. High cost and pernicious effects associated with the chemical and physical methods of nanoparticle synthesis are gradually paving the way for biological methods due to their eco-friendly nature. Considering the vast potentiality of microbes and plants as sources, biological synthesis can serve as a green technique for the synthesis of nanoparticles as an alternative to conventional methods. A number of reviews are available on green synthesis of nanoparticles but few have focused on covering the entire biological agents in this process. Therefore present paper describes the use of various living organisms like bacteria, fungi, algae, bryophytes and tracheophytes in the biological synthesis of metal nanoparticles, the mechanisms involved and the advantages associated therein.
Subject(s)
Green Chemistry Technology , Metal Nanoparticles/chemistry , Bacteria/chemistry , Bacteria/metabolism , Chlorophyta/chemistry , Chlorophyta/metabolism , Fungi/chemistry , Fungi/metabolism , Plant Extracts/chemistry , Plants/chemistry , Plants/metabolismABSTRACT
BACKGROUND: Assessment of genetic diversity in a crop-breeding programme helps in the identification of diverse parental combinations to create segregating progenies with maximum genetic variability and facilitates introgression of desirable genes from diverse germplasm into the available genetic base. RESULTS: In the present study, 39 strains of vegetable amaranth (Amaranthus tricolor) were evaluated for eight morphological and seven quality traits for two test seasons to study the extent of genetic divergence among the strains. Multivariate analysis showed that the first four principal components contributed 67.55% of the variability. Cluster analysis grouped the strains into six clusters that displayed a wide range of diversity for most of the traits. CONCLUSION: Cluster analysis has proved to be an effective method in grouping strains that may facilitate effective management and utilisation in crop-breeding programmes. The diverse strains falling in different clusters were identified, which can be utilised in different hybridisation programmes to develop high-foliage-yielding varieties rich in nutritional components.
Subject(s)
Amaranthus/genetics , Crops, Agricultural/genetics , Genetic Variation , Genotype , Nutritive Value , Phenotype , Vegetables/genetics , Amaranthus/classification , Biomass , Cluster Analysis , Crops, Agricultural/classification , Plant Leaves , Principal Component Analysis , Vegetables/classificationABSTRACT
Microsatellites are a ubiquitous class of simple repetitive DNA sequences, which are widespread in both eukaryotic and prokaryotic genomes. The use of microsatellites as polymorphic DNA markers has considerably increased both in the number of studies and in the number of organisms, primarily for genetic mapping, studying genomic instability in cancer, population genetics, forensics, conservation biology, molecular anthropology and in the studies of human evolutionary history. Although simple sequence repeats have been extensively used in studies encompassing varied areas of genetics, the mutation dynamics of these genome regions is still not well understood. The present review focuses on the mutational dynamics of microsatellite DNA with special reference to mutational mechanisms and their role in microsatellite evolution.
Subject(s)
Microsatellite Repeats , Mutation , Eukaryota , Evolution, Molecular , Genetic Markers , Humans , Models, GeneticABSTRACT
Populations in North India depend on a number of vegetable crops of which Amaranthus spp. is the most important since it is the only crop available in the hot summer months when no other foliage crop grows in the field. However, reports on mineral composition of leaves are rare with absolutely no information on the qualitative improvement of foliage yield with special reference to minerals. Studies on correlation among the minerals as well as with yield and leaf attributes are also lacking. Hence, we report the proximate mineral composition in 30 strains of A. tricolor along with some suggestions for qualitative improvement of the foliage yield with reference to minerals. Our study showed that vegetable amaranth is a rich source of minerals like calcium (1.7 +/- 0.04 g/100 g), iron (1233.8 +/- 50.02 mg/kg), and zinc (791.7 +/- 28.98 mg/kg). The heritability estimates were high for most of the traits, with potassium and calcium showing high values, while comparatively lower values were recorded for magnesium and nickel. Nickel was the only mineral that showed positive correlation with all the minerals, as well as with leaf size and foliage yield. Zinc showed strong positive relationship with iron (0.66**) and manganese (0.74**), and was the only mineral exhibiting significant positive association with foliage yield. This study would be of use in enhancement of selected minerals in different regions according to local preferences and nutrient deficiency prevalent among the populations.