Effects of municipal solid waste compost application on the microbial biomass of cultivated and non-cultivated soil in a semi-arid zone.

The aim of this study was to assess whether soil microbial biomass could be used as an indicator of environmental changes following the application of organic residue (compost of municipal solid waste and farmyard manure) or mineral fertilizers (N and P) into cultivated or uncultivated loam-clayey soil, for three consecutive years. The carbon and nitrogen of the microbial biomass (B(C) and B(N) were studied using the fumigation-extraction method. For the two cultivated and uncultivated plots, B(N) and B(C) were more important in the superficial profile (0-20 cm) than in the deep one (20-40 cm). In the uncultivated soil, we observed a good linear relation between B(C) and B(N) at the level of upper soil horizon during the wet season with r coefficients of 0.95, 0.71 and 0.80 for the consecutive years 2000, 2001 and 2002, respectively. Microbial biomasses C and N increased during the rainy season and decreased during the dry season. Microbial biomass C and N showed the higher content with compost and farmyard manure at 40 tonnes ha(-1). Moreover, the results showed that at the beginning of the experiment, the microbial biomass was higher in the ploughed wheat-cultivated plot than in the uncultivated one. Microbial biomass C and N in the cultivated plot amended with compost at 40 tonnes ha(-1) were significantly different in comparison with the soil microbial biomass amended with farmyard manure. The combining of chemical fertilizer with the organic fertilizer, such as compost at 40 or 80 tonnes ha(-1) and farmyard manure, increased the microbial biomasses C and N after 1 and 2 years. In the cultivated or uncultivated plots the results revealed that the best application rate of the compost was 40 tonnes ha(-1) and when the compost rate was increased from 40 to 80 tonnes ha(-1) both B(C) and B(N) decreased significantly.

Microbial C and N dynamics during composting process of urban solid waste.

Microbial characterization of composting is of importance for the optimization of the process and the quality of the end product. The aim of this work was to follow microbial biomass C and N dynamics during the composting process of urban solid waste. Microbial biomass C (B(C)) ranged from 4.06 to 1 microg kg(-1) of dry compost from day 5 to day 62 and decreased to reach 0.44 mg kg(-1) in mature compost. Microbial biomass N (B(N)) showed the same trend as B(C), ranging from 1.472 to 0.443 microg kg(-1) of dry compost from day 5 to day 62. This behaviour is probably related to the decreasing availability of readily decomposable substrates with the ongoing of the process. B(C) and B(N) showed a strong correlation (r = 0.78). The dynamics of the B(C)/B(N) ratio, index of the chemical composition of the whole microbial population suggested a shift in the composition of microbial populations during the process from prevailing bacteria and actinomycetes to prevailing fungi.