Nitrate assimilation pathway (NAP): role of structural (nit) and transporter (ntr1) genes in Fusarium oxysporum f.sp. lycopersici growth and pathogenicity.

The tomato pathogen Fusarium oxysporum f.sp. lycopersici possesses the capability to use nitrate as the only nitrogen source under aerobic and anaerobic conditions and to activate virulence-related functions when cultivated in the presence of nitrate, but not in ammonium. The genome of F. oxysporum f.sp. lycopersici encodes three paralogs nitrate reductase (NR) genes (nit1, nit2 and nit3) and one predicted ortholog of the Aspergillus nidulans high-affinity nitrate/nitrite transporters NtrA and NtrB, named ntr1. We set out to clarify the role of nit1, nit2, nit3 and ntr1 genes in nitrate assimilation and in the virulence of F. oxysporum f.sp. lycopersici. Quantitative RT-PCR analysis revealed that only nit1, nit2 and ntr1 are expressed at significant levels during growth in nitrate as the only nitrogen source. Targeted deletion of nit1 and ntr1, but not of nit2 or nit3, severely impaired growth of F. oxysporum on nitrate as nitrogen source, indicating that Nit1 and Ntr1 proteins are involved in nitrate assimilation by the fungus; biochemical analysis of nit mutants indicated that Nit1 and Nit2 enzymes contribute to about 50 and 30% of the total NR activity, respectively. In addition, a spontaneous chlorate-resistant mutant derived from F. oxysporum 4287, denoted NitFG, was characterized, showing inability to grow in nitrate under aerobic and anaerobic conditions and low levels of NR activity, in spite of its increased transcription levels of nit1 and nit2 genes. Tomato plant infection assays showed that NitFG and ∆ntr1 mutants induced an earlier death in tomato plants, whereas the single mutants ∆nit1, ∆nit2 and ∆nit1∆nit2 double mutant showed a mortality rate similar to the wild-type strain. Taken together, these results indicate that the Nit1 and Ntr1 proteins are important for nitrate assimilation of F. oxysporum f.sp. lycopersici incubated under aerobic and anaerobic conditions and that this metabolic process is not essential for the virulence of the fungus. These observations open new questions about the role of Nit1, Nit2, and Nit3 proteins in other routes of nitrate metabolism in this pathogenic fungus and in the possible regulatory role that can be exerted by the AreA protein in these routes.

Factors associated with late fetal mortality.

PURPOSE: Perinatal mortality has been decreasing in Europe thanks to a reduction in neonatal mortality. The causes of fetal mortality remain poorly studied. The objective was to determine the late fetal mortality rate in Spain in 2015 and the associated factors. METHODS: A cross-sectional study was performed using data regarding births in 2015 in Spain extracted from the National Institute of Statistics. Single births at 28 or more weeks of pregnancy were included. The sample comprised 340,371 births. Sociodemographic, obstetrical and neonatal variables were analyzed using univariate and multivariate logistic regression (MLR), with the fetal mortality from 28 weeks of pregnancy as the dependent variable. RESULTS: The total number of late fetal deaths was 884 (2.6 × 1000). The MLR model showed that the following factors were associated with late fetal mortality: birth before 37 weeks of pregnancy (OR 13.1); weight of the newborn < 2500 g (OR 3.22) and ≥ 4000 g (OR 3.36); low training level (OR 2.28); and others, such as African origin, maternal age ≥ 35 years, primiparity and mothers who were single. CONCLUSIONS: The rate of late fetal mortality in Spain has not decreased and has remained at the same level as in 2010. This result is related to prematurity, low birth weight, macrosomia and sociodemographic factors, such as low maternal preparation, mothers of African origin, age ≥ 35 years and mothers who are single. It is necessary to improve the quality and accessibility of prenatal care and the early detection of risk factors.

Aplicación de la teoría de aprendizaje en el entrenamiento acerca de seguridad y salud para trabajadores inmigrantes en las lecherías.

Resumen Con el propósito de prevenir lesiones y enfermedades ocupacionales, diseñamos un currículo de seguridad y salud para trabajadores inmigrantes en la industria lechera para aumentar el conocimiento, fomentar prácticas de seguridad, y reducir inequidades en la comunicación. El currículo se basa principalmente en la Taxonomía para el Aprendizaje Significativo-TAS ( Taxonomy of Significant Learning) e incorpora teorías de la conducta y de aprendizaje para adultos, así como también los principios de control de riesgos ocupacionales. Los entrenamientos se implementaron con 836 trabajadores de habla hispana de 67 lecherías en el estado de Wisconsin, en los Estados Unidos. El 67% de los trabajadores reportaron nunca haber recibido entrenamiento acerca de la seguridad en las lecherías, el 65% reportó haber trabajado en lecherías durante 5 años o menos, y el 26% de los trabajadores reportaron haber sufrido alguna lesión mientras trabajaban en la lechería. La evaluación cuantitativa y cualitativa de los entrenamientos sugiere que nuestro currículo efectivamente aumentó el conocimiento y fomentó la contemplación de prácticas de seguridad de los trabajadores. El aumento del conocimiento en general del 25% es estadísticamente significativo (p < .01). Los trabajadores recordaron al menos un concepto clave del contenido, expresaron sentirse confiados en adoptar al menos una conducta de seguridad, y mencionaron su intención de comunicar sus preocupaciones de seguridad a sus jefes en la lechería. De acuerdo a nuestro conocimiento, esta es la primera vez que se aplica la TAS en la educación acerca de seguridad y salud ocupacional. Este currículo puede ayudar a los productores en la industria lechera a cumplir con el entrenamiento anual de los trabajadores requerido por la Administración de Seguridad y Salud Ocupacional de los E. U. ( Occupational Safety and Health Administration-OSHA), ofreciendo este entrenamiento básico en seguridad y salud a sus trabajadores durante su etapa inicial de empleo.

Applying Learning Theory to Safety and Health Training for Hispanic Immigrant Dairy Workers.

We designed a safety and health curriculum for dairy immigrant workers aiming to increase knowledge, encourage safe behavior, and reduce worker communication inequalities to prevent occupational injury and diseases. The design is largely based on the Taxonomy of Significant Learning and incorporated behavioral and adult learning theories and principles of occupational hazard control. Trainings were implemented with 836 Spanish-speaking workers from 67 farms in Wisconsin. Sixty-seven percent of workers reported never being trained before in dairy safety, 65% of these worked in dairy for 5 or fewers years, and 26% of workers reported being ever injured while working on dairy. Quantitative and qualitative evaluation of the trainings suggest that our curriculum successfully increased worker knowledge and promoted contemplation of safe practices. The overall knowledge gain of 25% was statistically significant ( p < .01). Workers recalled at least one key concept, expressed confidence of adopting at least one safety behavior, and mentioned their intention to communicate safety concerns to farmers. To our knowledge, this is the first Taxonomy of Significant Learning application to occupational safety and health education. Our curriculum can support dairy farmers' compliance with Occupational Safety and Health Administration's annual training requirements by providing our basic safety and health training to workers at early job stages.

Candida glabrata encodes a longer variant of the mating type (MAT) alpha2 gene in the mating type-like MTL3 locus, which can form homodimers.

The fungal pathogen Candida glabrata is a haploid asexual yeast. Candida glabrata contains orthologs of the genes that control mating and cell-type identity in other fungi, which encode putative transcription factors localized in the MAT locus in Saccharomyces cerevisiae or MTL in other fungi. Candida glabrata contains three copies of the CgMTL locus but only CgMTL1 correctly expresses the information encoded in it. CgMTL1 can encode the Cg A1: gene ( A: information), or the Cgalpha1 and Cgalpha2 genes (alpha information). CgMTL2 contains an identical copy of the Cg A1: gene. CgMTL3 contains an identical copy of the Cgalpha1 gene but a longer variant of the Cgalpha2 gene that we termed Cgalpha3. In S. cerevisiae diploid cells, that express Sc A: and Scalpha information, Sc A1: and Scalpha2 proteins form a heterodimer, which represses genes expressed only in haploid cells and some genes involved in stress response. We constructed C. glabrata strains that simultaneously express Cg A1: and Cgalpha2 or Cg A1: and Cgalpha3 genes. We did not find any phenotype in these strains when grown under a large variety of stress and nutritional conditions. However, we detected an interaction between Cg A1: and Cgalpha2 but not between Cg A1: and Cgalpha3 by Bimolecular Fluorescence Complementation and co-immunoprecipitation assays.

Immigrant dairy workers' perceptions of health and safety on the farm in America's Heartland.

BACKGROUND: Dairy farming is dangerous. Yearly, farms grow fewer and larger by employing immigrant workers, who have limited industrial agriculture experience and safety and health training. METHODS: We examined results of five focus groups with 37 Hispanic, immigrant dairy workers. Analysis followed a grounded theory approach and employed ATLAS.ti. RESULTS: Reported injury experience affirmed the hazardous nature of dairy. Some workers received appropriate worker compensation benefits, whereas others were instructed to deny work-relatedness. Some employers covered medical injury costs out-of-pocket, whereas others did not. Cows were a major injury source. Pressure to work and weather were noted as injury risk factors. Worker compensation was poorly understood, and immigration status and fear of deportation influenced injury and hazard reporting. CONCLUSION: Injury management practices range from benevolent to threatening. Workers compensation is poorly understood and undocumented status is an occupational hazard. We underscore the need for further research and immigration policy change.

Expression vectors for C-terminal fusions with fluorescent proteins and epitope tags in Candida glabrata.

Candida glabrata is a haploid yeast considered the second most common of the Candida species found in nosocomial infections, accounting for approximately 18% of candidemias worldwide. Even though molecular biology methods are easily adapted to study this organism, there are not enough vectors that will allow probing the transcriptional and translational activity of any gene of interest in C. glabrata. In this work we have generated a set of expression vectors to systematically tag any gene of interest at the carboxy-terminus with three different fluorophores (CFP, YFP and mCherry) or three epitopes (HA, FLAG or cMyc) independently. This system offers the possibility to generate translational fusions in three versions: under the gene's own promoter integrated in its native locus in genome, on a replicative plasmid under its own promoter, or on a replicative plasmid under a strong promoter to overexpress the fusions. The expression of these translational fusions will allow determining the transcriptional and translational activity of the gene of interest as well as the intracellular localization of the protein. We have tested these expression vectors with two biosynthetic genes, HIS3 and TRP1. We detected fluorescence under the microscope and we were able to immunodetect the fusions using the three different versions of the system. These vectors permit coexpression of several different fusions simultaneously in the same cell, which will allow determining protein-protein and protein-DNA interactions. This set of vectors adds a new toolbox to study expression and protein interactions in the fungal pathogen C. glabrata.

Novel Adiposity and Biochemical-Anthropometric Indices to Identify Cardiometabolic Risk and Metabolic Syndrome in Mexican Adults.

Although several indices used in clinical practice identify cardiometabolic risk (CR) and metabolic syndrome (MetS), it is imperative to develop indices for specific populations. Therefore, we proposed and validated sex-specific indices to identify CR associated with visceral adipose tissue (VAT) accumulation or MetS in Mexican adults. Additionally, a cut-off value for the visceral fat area (VFA) to identify CR was proposed. Clinical, anthropometric, biochemical, and body composition variables were evaluated in 904 subjects (25-45 years old) (84.4% men). Multiple and logistic regressions were used to model the indices and ROC curve analysis to determine predictive performance. An additional cohort (n = 186) was used for indices validation, and Cohen's kappa coefficient was employed for agreement analysis. The proposed sex-specific indices, called Mexican adiposity indices (MAIs) and biochemical-anthropometric indices (BAIs), were good predictors for CR and MetS. The kappa coefficients showed a moderate agreement level. The VFA cut-off value chosen to identify CR was 100.3 cm2 because it had the best combination of sensitivity (66.8%) and specificity (64.4%). MAIs and BAIs could be clinical tools to identify either CR associated to VAT accumulation or MetS, respectively. A VFA cut-off value of 100.3 cm2 could identify CR in Mexican men.

[Surveillance of rare diseases in Spain: Spanish Registry of Rare Diseases (ReeR).] / Vigilancia de las enfermedades raras en España: el Registro Estatal de Enfermedades Raras (ReeR).

Traditionally, epidemiological surveillance has focused on infectious diseases, but the concept of Public Health surveillance, introduced in Spain with the Law 33/2011, is broader and includes chronic diseases. Health strategies for these diseases need epidemiological information to improve understanding of socio-health needs and to facilitate the efficient management of resources. The European Union defines rare diseases (RD) as those that, being life-threatening or chronically debilitating, have a prevalence of less than 5 cases per 10,000 inhabitants. The RD Strategy of the National Health System, approved in 2009 and updated in 2014, recommends the development of regional registries of rare diseases (RAER), in addition to a national registry. The REpIER and Spain-RDR projects of the Institute of Health Carlos III (ISCIII) promoted the creation and regulation of 94% of the RAER. After more than 10 years of initiatives and work to improve the knowledge of RD's epidemiology in Spain, it was possible to implement the Spanish Registry of Rare Diseases (ReeR) in 2015, becoming one of the first population surveillance systems for chronic diseases of state scope. The ReeR procedures manual is the result of consensus between the RAER, the Ministry of Health, the ISCIII and the patient associations. The participatory methodology used for the implementation and launching of ReeR is considered an added value. The information system implemented will allow improving knowledge about the prevalence and distribution of RD in Spain.

Tradicionalmente la vigilancia epidemiológica se ha centrado en enfermedades transmisibles, pero el concepto de vigilancia en Salud Pública, incorporado en España con la Ley 33/2011, es más amplio e incluye las enfermedades crónicas. Las estrategias de salud para estas enfermedades necesitan disponer de información epidemiológica para mejorar el conocimiento de las necesidades sociosanitarias y facilitar la gestión eficiente de recursos. La Unión Europea define las enfermedades raras (ER) como aquellas que, con peligro de muerte o invalidez crónica, presentan una prevalencia inferior a 5 casos por cada 10.000 habitantes. La Estrategia en ER del Sistema Nacional de Salud, aprobada en 2009 y actualizada en 2014, recomienda desarrollar registros autonómicos de enfermedades raras (RAER) y uno estatal. Los proyectos REpIER y Spain-RDR del Instituto de Salud Carlos III (ISCIII) impulsaron la creación y regulación del 94% de los RAER; y tras más de 10 años de iniciativas y trabajos para mejorar el conocimiento de la epidemiología de las ER en España, se logró implementar el Registro Estatal de Enfermedades Raras (ReeR) en 2015, convirtiéndose en uno de los primeros sistemas de vigilancia poblacional de enfermedades crónicas de ámbito estatal. El manual de procedimientos del ReeR es el resultado del consenso entre los RAER, Ministerio de Sanidad, ISCIII y asociaciones de pacientes. La metodología participativa empleada para la implementación y puesta en funcionamiento del ReeR es considerada un valor añadido. El sistema de información implementado va a permitir mejorar el conocimiento sobre la prevalencia y distribución de las ER en España.

Three-dimensional synaptic organization of the human hippocampal CA1 field.

The hippocampal CA1 field integrates a wide variety of subcortical and cortical inputs, but its synaptic organization in humans is still unknown due to the difficulties involved studying the human brain via electron microscope techniques. However, we have shown that the 3D reconstruction method using Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) can be applied to study in detail the synaptic organization of the human brain obtained from autopsies, yielding excellent results. Using this technology, 24,752 synapses were fully reconstructed in CA1, revealing that most of them were excitatory, targeting dendritic spines and displaying a macular shape, regardless of the layer examined. However, remarkable differences were observed between layers. These data constitute the first extensive description of the synaptic organization of the neuropil of the human CA1 region.

There are billions of nerve cells or neurons in the human brain, and each one can form thousands of connections, also called synapses, with other neurons. That means there are trillions of synapses in the brain that keep information flowing. Studying the arrangement of individual neurons in the human brain, and the connections between them, is incredibly difficult because of its complexity. Scientists have tools that can image the whole brain and can measure the activity in different regions, but these tools only visualize brain structures that are large enough to be seen with human eyes. Synapses are much smaller (in the range of nanometers), and can only be seen using thin slices of preserved brain tissue through a technique called electron microscopy. The hippocampus is a part of the human brain that is critical for memory, learning and spatial orientation, and is affected in epilepsy and Alzheimer's disease. Although numerous studies of the hippocampus have been performed in laboratory animals, such as mice, the question remains as to how much of the information gained from these studies applies to humans. Thus, studying the human brain directly is a major goal in neuroscience. However, the scarcity of human brain tissue suitable for the study of synapses is one of the most important issues to overcome. Fortunately, healthy human brain tissue that can be studied using electron microscopy is sometimes donated after death. Using these donations could improve the understanding of the synapses in normal brains and possible changes associated with disease. Now, Montero-Crespo et al. have mapped synapses in the normal human hippocampus in three dimensions ­ providing the first detailed description of synaptic structure in this part of the brain. Using high-powered electron microscopes and donated brain tissue samples collected after death, Montero-Crespo et al. imaged almost 25,000 connections between neurons. The analysis showed that synapses were more densely packed in some layers of the hippocampus than in others. Most synapses were found to be connected to tiny dendritic 'spines' that sprout from dendritic branches of the neuron, and they activated (not suppressed) the next neuron. Beyond its implications for better understanding of brain health and disease, this work could also advance computer modelling attempts to mimic the structure of the brain and its activity.

Mujeres con elevado nivel de estudios, víctimas del cuidado de personas con Alzheimer / Women with high level of education, victims of the care of people with Alzheimer's

OBJETIVO: Conocer la relación existente entre la tarea de cuidar a pacientes con Enfermedad de Alzheimer, el Género de los cuidadores y su nivel de formación académica. MÉTODO: Estudio descriptivo, como sujetos de estudio 69 personas diagnosticadas de Enfermedad de Alzheimer y sus respectivos cuidadores familiares. En los cuidadores se analizó: la edad, el género, el nivel de estudios, carga, depresión, nivel de ansiedad, calidad de vida y soledad social. En los pacientes se valoró la edad, género, nivel de dependencia y estado neuropsicológico. La recogida de datos se realizó en 2016. Entre otros análisis se realizó análisis de regresión logística. RESULTADOS: Se obtuvo que los cuidadores con estudios superiores sufrían mayor carga, siendo mujeres en su mayoría. Mujeres con más probabilidad de presentar soledad social, altos niveles de ansiedad y peor calidad de vida que los hombres. La carga puede ser debida al gran número de responsabilidades y a la imposibilidad de combinarlas con el papel de cuidador. Se puede concluir que las mujeres llegan a ser víctimas del cuidado de personas con Enfermedad de Alzheimer

The aim of this job is to know the existing relationship between the task of caring patients with Alzheimer's disease, caregivers' gender and their level of academic studies. Descriptive study, 69 persons diagnosed with Alzheimer's disease and their respective familiar caregivers as subjects of the study. Age, gender, academic level, burden, depression, anxiety level, quality of life and social loneliness have been measured in caregivers. Age, gender, dependency level and neuropsychological state have been measured in patients. Data collection was done in 2016. Logistic regression analysis was performed. Caregivers with high levels of academic studies suffer more burden, being women in their majority. They are more likely to present social loneliness and higher levels of anxiety and a worse quality of life than men. The burden may be due to a greater number of responsibilities to respond to, and to the inability to combine it with the role of caregiver. We can conclude that women become victims of caring Alzheimer' patients

The use of audience response system technology with limited-english-proficiency, low-literacy, and vulnerable populations.

Audience response systems (ARS) have long been used to improve the interactivity of educational activities. Most studies of ARS have addressed education of literate trainees. How well these devices work with low-literacy subjects is not well studied. Information gathering on the training audience is an important use of ARS and helpful in improving the targeting of training information. However, obtaining demographic information from vulnerable populations with reasons to be concerned about divulging information about themselves has not been tested. In addition, a culturally competent method to effectively collect demographic and evaluation data of this growing population is essential. This project investigated the use of ARS to gather information from Hispanic immigrant workers, many of whom are socially vulnerable and have limited English proficiency (LEP) and low-literacy. Workers attended focus groups and were asked to use ARS devices or clickers to respond to questions. Questions were both categorical (multiple choice) and open-ended numerical (text entry), and varied from simple queries to more sensitive points regarding immigration. Most workers answered the one-key response categorical questions with little difficulty. In contrast, some participants struggled when responding to numerical questions, especially when the response required pressing multiple clicker keys. An overwhelming majority of participants reported that the clickers were comfortable and easy to use despite the challenges presented by the more complex responses. The error rate increased as question complexity increased and the trend across three ordered categories of response complexity reached statistical significance. Results suggest that ARS is a viable method for gathering dichotomous or higher-order categorical information from LEP and low-literacy populations in a group setting while assuring anonymity. However, it is recommended that clickers be developed and tested with fewer, bigger, and more widely separated buttons, and less printing on the buttons for these populations. Further research is needed to determine the effectiveness of using clickers with simplified configurations in the workplace as a tool to collect data for surveys and assessments and to better engage LEP and low-literacy workers in training sessions.

The mating type-like loci of Candida glabrata.

Candida glabrata, a haploid and opportunistic fungal pathogen that has not known sexual cycle, has conserved the majority of the genes required for mating and cell type identity. The C. glabrata genome contains three mating-type-like loci called MTL1, MTL2 and MTL3. The three loci encode putative transcription factors, a1, α1 and α2 that regulate cell type identity and sexual reproduction in other fungi like the closely related Saccharomyces cerevisiae. MTL1 can contain either a or α information. MTL2, which contains a information and MTL3 with α information, are relatively close to two telomeres. MTL1 and MTL2 are transcriptionally active, while MTL3 is subject to an incomplete silencing nucleated at the telomere that depends on the silencing proteins Sir2, Sir3, Sir4, yKu70/80, Rif1, Rap1 and Sum1. C. glabrata does not seem to maintain cell type identity, as cell type-specific genes are expressed regardless of the type (or even absence) of mating information. These data highlight important differences in the control of mating and cell type identity between the non-pathogenic yeast S. cerevisiae and C. glabrata, which might explain the absence of a sexual cycle in C. glabrata. The fact that C. glabrata has conserved the vast majority of the genes involved in mating might suggest that some of these genes perhaps have been rewired to control other processes important for the survival inside the host as a commensal or as a human pathogen. This manuscript is part of the series of works presented at the "V International Workshop: Molecular genetic approaches to the study of human pathogenic fungi" (Oaxaca, Mexico, 2012).

The mating type-like loci of Candida glabrata / Los loci de apareamiento de Candida glabrata

Candida glabrata, a haploid and opportunistic fungal pathogen that has not known sexual cycle, has conserved the majority of the genes required for mating and cell type identity. The C. glabrata genome contains three mating-type-like loci called MTL1, MTL2 and MTL3. The three loci encode putative transcription factors, a1, α1 and α2 that regulate cell type identity and sexual reproduction in other fungi like the closely related Saccharomyces cerevisiae. MTL1 can contain either a or α information. MTL2, which contains a information and MTL3 with α information, are relatively close to two telomeres. MTL1 and MTL2 are transcriptionally active, while MTL3 is subject to an incomplete silencing nucleated at the telomere that depends on the silencing proteins Sir2, Sir3, Sir4, yKu70/80, Rif1, Rap1 and Sum1. C. glabrata does not seem to maintain cell type identity, as cell type-specific genes are expressed regardless of the type (or even absence) of mating information. These data highlight important differences in the control of mating and cell type identity between the non-pathogenic yeast S. cerevisiae and C. glabrata, which might explain the absence of a sexual cycle in C. glabrata. The fact that C. glabrata has conserved the vast majority of the genes involved in mating might suggest that some of these genes perhaps have been rewired to control other processes important for the survival inside the host as a commensal or as a human pathogen. This manuscript is part of the series of works presented at the "V International Workshop: Molecular genetic approaches to the study of human pathogenic fungi" (Oaxaca, Mexico, 2012) (AU)

Candida glabrata, una levadura patógena haploide y oportunista, que carece de ciclo sexual conocido (asexual), conserva la mayoría de genes ortólogos requeridos en los procesos de apareamiento, esporulación y la identidad del tipo celular. El genoma de C. glabrata contiene 3 loci de apareamiento llamados MTL1, MTL2 y MTL3 que codifican los presuntos factores de transcripción a1, α1 y α2 que controlan la reproducción sexual e identidad celular en otros hongos, como Saccharomyces cerevisiae con el cual tiene una estrecha relación filogenética. MTL1 puede contener información a o α; MTL2 contiene información a, y MTL3 que contiene información α1 y α2 son loci próximos a 2 telómeros. MTL1 y MTL2 son activos transcripcionalmente mientras que MTL3 está sujeto a un silenciamiento que no es completo, que proviene del telómero y depende de las proteínas Sir2, Sir3, Sir4, yKu70/80, Rif1, Rap1 y Sum1. C. glabrata parece no mantener identidad de tipo celular ya que varios genes específicos de un tipo celular se expresan en todas las células con independencia del tipo de información de apareamiento en los loci MTL, o incluso, en su ausencia. Estos datos ilustran varias diferencias importantes entre la levadura no patógena S. cerevisiae y C. glabrata que podrían explicar la característica asexual en esta última. El hecho de que en C. glabrata se hayan conservado los genes necesarios para el apareamiento podría indicar que es posible que algunos de estos genes se hayan «reorganizado» para controlar otros procesos importantes en la supervivencia de C. glabrata en su huésped, como comensal o como patógeno.Este artículo forma parte de una serie de estudios presentados en el «V International Workshop: Molecular genetic approaches to the study of human pathogenic fungi» (Oaxaca, México, 2012) (AU)

Realising a resilient and sustainable built environment: towards a strategic agenda for the United Kingdom.

Recent natural and human-induced emergencies have highlighted the vulnerability of the built environment. Although most emergency events are not entirely unexpected, and the effects can be mitigated, emergency managers in the United Kingdom have not played a sufficiently proactive role in the mitigation of such events. If a resilient and sustainable built environment is to be achieved, emergency management should be more proactive and receive greater input from the stakeholders responsible for the planning, design, construction and operation of the built environment. This paper highlights the need for emergency management to take a more systematic approach to hazard mitigation by integrating more with professions from the construction sector. In particular, design changes may have to be considered, critical infrastructures must be protected, planning policies should be reviewed, and resilient and sustainable agendas adopted by all stakeholders.

Study of the anti-inflammatory effect of Sedum praealtum (Siempreviva) in the rat: dose-dependent response.

The results obtained suggest that the siempreviva has an anti-inflammatory effect and that the response was dose-dependent. The lyophilized siempreviva, at the doses used in this study, is not toxic.

La fenomenología de la comorbilidad del trauma y la psicosis / The phenomenology of the comorbidity of trauma and psychosis

A lo largo de este artículo queremos reflejar cómo la psicosis y el trastorno porestrés postraumático (TEPT) no son entidades tan distintas ni separadas, sino que confrecuencia confluyen y se solapan. Muchas investigaciones nos indican que la prevalenciavital de TEPT en pacientes mentales graves es muy superior a la de la poblacióngeneral. Algunos autores concluyen que algunas personas con sintomatología psicóticadesarrollan TEPT como resultado de la propia experiencia psicótica. Durante muchosaños los investigadores se han centrado preferentemente en la relación del trauma consíndromes no psicóticos. No obstante, en los últimos años se ha producido un gran interéssobre cómo el trauma puede precipitar o favorecer la aparición de cuadros psicóticos.Por otro lado, parece existir evidencia empírica que señala la existencia de un subtipoTEPT con síntomas psicóticos. Por último, Morrison, Frame y Larkin (2003) plantean,que tanto la psicosis como el TEPT, representan un continuo de respuestas del individuosometido a un evento traumático. Las similitudes entre ambos trastornos indican quepodrían formar parte del mismo espectro de respuestas ante un acontecimiento traumático.No obstante, es preciso indagar el por qué el trauma da lugar un tipo de síntomas uotros. Visto todo lo anterior, el objetivo de este artículo es examinar la naturaleza de lasdistintas relaciones entre la psicosis y el trauma. Así como, recomendar encarecidamenteque se introduzcan protocolos específicos y preguntas rutinarias para detectar y tratar elabuso sexual y físico en pacientes con trastornos psicóticos

Throughout this article we have attempted to show how psychotic disorders and posttraumatic stress disorder (PTSD) are not such distinct or apart entities, on the contrary they frequently come together and overlap. A lot of research has indicated that the vital prevalence of PTSD in severe mental illness is quite superior to that of the general population. Some authors has concluded that some people with psychotic symptoms develop PTSD due to their own psychotic experience. During many years researches have focused on the relation of trauma with non psychotic syndromes. Nonetheless, in recent years a great interest has been raised on how trauma might precipitate the onset of psychosis. Moreover, there is empirical evidence that points out to the existence of PTSD subtype with psychotic symptomatology. Finally, Morrison, Frame y Larkin (2003) proposed that psychosis as well as PTSD represent a continuum of responses of an individual exposed to a traumatic event. The similarities among both disorders indicate that they could form part of a spectrum of post-traumatic responses. However, it is necessary to ascertain why trauma produces a type of symptom or the other. Consequently, the aim of the article is to examine the nature of the different relations between psychosis and trauma. Additionally, it is to recommend earnestly the introduction of specific protocols and routine inquiries to detect and treat sexual and physical abuse for persons with psychotic disorders

Manual organizativo del servicio de alimentación hospitalario: docucmento de trabajo

Presenta el Manual Organizativo del servicio de alimentación hospitalario, elaborado de acuerdo a resultados y diagnóstico realizado en los servicios de alimentación de 32 hospitales del país. El manual contiene políticas, normas, procedimientos e insturmentos de trabajo necesarios para el buen funcionamiento de los servicios de alimentación. Enfoca las cinco etapas del proceso administrativo que le permite al personal que dirije los servicios de alimentación integrar eficientemente la gestión administrativa y dar a los pacientes y personal hospitario un mejor servicio