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1.
Curr Microbiol ; 76(8): 962, 2019 08.
Article in English | MEDLINE | ID: mdl-29279979

ABSTRACT

The original version of this article unfortunately contained a mistake. The legends of Tables 2 and 3, Fig. 1 are incorrect. The corrected legends are given below.

2.
Curr Microbiol ; 74(2): 203-211, 2017 Feb.
Article in English | MEDLINE | ID: mdl-27942843

ABSTRACT

Extended-spectrum, ß-lactamase-producing Escherichia coli (ESBL-E) harboring the bla CTX-M-55-encoding plasmid (ESBL-E55) has been reported to be associated with urinary tract infection (UTI). The aims of this study were to clarify the prevalence of ESBL-E55 in pork meats and workers from the same wholesale market, as well as patients with UTI from a nearby hospital in Vietnam; we also investigated the plasmids encoding bla CTX-M-55. Sequencing analysis showed that 66.6% of the ESBL-E isolated from pork meats contained bla CTX-M-55, whereas the gene was present in 25.0% of workers and 12.5% of patients with UTI. Plasmid analysis showed that several sizes of plasmid encoded bla CTX-M-55 in ESBL-E55 isolated from pork meats, whereas ESBL-E55 isolated from workers and patients with UTI contained only 104-139 kbp of bla CTX-M-55-encoding plasmids. This indicates that the 104-139 kbp sizes of bla CTX-M-55-encoding plasmids were commonly disseminated in pork meats, wholesale market workers, and patients with UTI.


Subject(s)
Carrier State/microbiology , Escherichia coli Infections/microbiology , Escherichia coli Proteins/genetics , Escherichia coli/enzymology , Meat/microbiology , Plasmids/analysis , Urinary Tract Infections/microbiology , beta-Lactamases/genetics , Escherichia coli/genetics , Escherichia coli/isolation & purification , Escherichia coli Infections/epidemiology , Humans , Molecular Epidemiology , Prevalence , Sequence Analysis, DNA , Vietnam/epidemiology
3.
Sci Rep ; 7: 39997, 2017 01 05.
Article in English | MEDLINE | ID: mdl-28054672

ABSTRACT

We report the quantum efficiency of photoluminescence processes of Er optical centers as well as the thermal quenching mechanism in GaN epilayers prepared by metal-organic chemical vapor deposition. High resolution infrared spectroscopy and temperature dependence measurements of photoluminescence intensity from Er ions in GaN under resonant excitation excitations were performed. Data provide a picture of the thermal quenching processes and activation energy levels. By comparing the photoluminescence from Er ions in the epilayer with a reference sample of Er-doped SiO2, we find that the fraction of Er ions that emits photon at 1.54 µm upon a resonant optical excitation is approximately 68%. This result presents a significant step in the realization of GaN:Er epilayers as an optical gain medium at 1.54 µm.

4.
Ann Chir ; 51(8): 848-53, 1997.
Article in French | MEDLINE | ID: mdl-9734093

ABSTRACT

A new system based on 3D digitization with magnetic fields has been developed by our research team. The focus of this study was to evaluate the intratester reproducibility of this technique of measurement. Twenty key morphological parameters were used twice to digitize the fourty five female subjects aged 7 to 23 years. The results of variance analysis (ANOVA) for repeated measures showed no statistically significant difference between the two series of measurements for the twenty angles studied. In 56% of the measurements, the difference of the means was less than 1 degree, the greatest being 1.82 degrees. These results confirm the reliability of this new technique of postural evaluation when the measurements are taken by the same tester. Therefore, 3D digitization with magnetic fields could be an interesting alternative to X-Rays for the evaluation of scoliosis.


Subject(s)
Numerical Analysis, Computer-Assisted , Posture , Adolescent , Adult , Analysis of Variance , Child , Data Interpretation, Statistical , Female , Humans , Observer Variation , Scoliosis/diagnosis , Spinal Curvatures/physiopathology
5.
Virology ; 350(2): 258-68, 2006 Jul 05.
Article in English | MEDLINE | ID: mdl-16713612

ABSTRACT

Highly pathogenic avian influenza virus H5N1 is endemic in poultry in East and Southeast Asia with disease outbreaks recently spreading to parts of central Asia, Europe and Africa. Continued interspecies transmission to humans has been reported in Vietnam, Thailand, Cambodia, Indonesia and China, causing pandemic concern. Here, we genetically characterize 82 H5N1 viruses isolated from poultry throughout Indonesia and Vietnam and 11 human isolates from southern Vietnam together with sequence data available in public databases to address questions relevant to virus introduction, endemicity and evolution. Phylogenetic analysis shows that all viruses from Indonesia form a distinct sublineage of H5N1 genotype Z viruses suggesting this outbreak likely originated from a single introduction that spread throughout the country during the past two years. Continued virus activities in Indonesia were attributed to transmission via poultry movement within the country rather than through repeated introductions by bird migration. Within Indonesia and Vietnam, H5N1 viruses have evolved over time into geographically distinct groups within each country. Molecular analysis of the H5N1 genotype Z genome shows that only the M2 and PB1-F2 genes were under positive selection, suggesting that these genes might be involved in adaptation of this virus to new hosts following interspecies transmission. At the amino acid level 12 residues were under positive selection in those genotype Z viruses, in the HA and PB1-F2 proteins. Some of these residues were more frequently observed in human isolates than in avian isolates and are related to viral antigenicity and receptor binding. Our study provides insight into the ongoing evolution of H5N1 influenza viruses that are transmitting in diverse avian species and at the interface between avian and human hosts.


Subject(s)
Influenza A Virus, H5N1 Subtype/physiology , Influenza A Virus, H5N1 Subtype/pathogenicity , Influenza in Birds/epidemiology , Influenza, Human/epidemiology , Animals , Asia, Southeastern , Birds , Disease Outbreaks , Humans , Indonesia , Influenza A Virus, H5N1 Subtype/classification , Influenza A Virus, H5N1 Subtype/genetics , Molecular Sequence Data , Phylogeny , Vietnam/epidemiology , Zoonoses
6.
Am J Respir Crit Care Med ; 155(6): 1841-7, 1997 Jun.
Article in English | MEDLINE | ID: mdl-9196084

ABSTRACT

Atopy, specific IgE sensitization, and bronchial hyperresponsiveness (BHR) were examined in a cohort of 769 apprentices starting career programs in animal health or veterinary medicine (Group 1), pastry making (Group 2), and dental hygiene (Group 3). The hypothesis were that: (1) a proportion of subjects can be "sensitized" although no significant specific occupational exposure has occurred; and (2) there is a relationship between baseline specific sensitization to work-related antigens and host characteristics. Skin tests were administered using 11 common inhalants and specific allergens, including six laboratory animal extracts, three cereal antigens, alpha-amylase, and latex. Methacholine challenge tests were performed. The prevalence of atopy was 54.4% in Group 1, 58.1% in Group 2, and 52.5% in Group 3. Skin reactivity to work-specific proteins was as follows: laboratory animal proteins, 13.8% in Group 1, 14.0% in Group 2, and 15.6% in Group 3. No subject was sensitized to alpha-amylase, whereas 1.2% in Group 1, 5% in Group 2, and 4.1% in Group 3 were sensitized to flour. Five subjects reacted to latex. BHR (PC20 < or = 8 mg/ml) was present in 17.6%, 21.2%, and 14.8% of subjects in Groups 1, 2, and 3, respectively. Specific sensitization was associated with positive skin reactions to common allergens, work-related symptoms, and BHR. These results suggest that students starting career programs with exposure to high-molecular-weight allergens have a low but substantial frequency of specific sensitization to work-related allergens that is related to atopy and BHR.


Subject(s)
Allergens/immunology , Bronchial Hyperreactivity/immunology , Environmental Exposure , Hypersensitivity/immunology , Immunization , Immunoglobulin E/immunology , Students, Medical , Students , Adult , Asthma/epidemiology , Dental Hygienists/education , Education, Veterinary , Female , Humans , Male , Prevalence , Skin Tests
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